Effect of NaHS on carbonic anhydrase activity of human erythrocyte

Authors

  • Abhijit Bhakta Associate Professor, Department of Anantomy, Nilratan Sircar Medical College and Hospital, Kolkata
  • Maitreyi Bandyopadhyay Associate Professor, Department of Microbiology, R G Kar Medical College Kolkata
  • Sayantan Dasgupta Assistant Professor, Department of Biochemistry, Nilratan Sircar Medical College and Hospital, Kolkata http://orcid.org/0000-0002-8483-7072
  • Santanu Sen Assistant Professor, Department of Biochemistry, Nilratan Sircar Medical College and Hospital, Kolkata http://orcid.org/0000-0003-1964-9005
  • Arun Kumar Professor, Department of Biochemistry, Shri Shankaracharya Institute of Medical Sciences, Bhilai, Chhattisgarh http://orcid.org/0000-0002-8800-0296
  • Utpal Kumar Biswas Professor and Head, Department of Biochemistry, Nilratan Sircar Medical College and Hospital, Kolkata http://orcid.org/0000-0002-4714-0065

DOI:

https://doi.org/10.3126/ajms.v7i3.14047

Keywords:

H2S, Carbonic Anhydrase Activity, NaHS, erythrocytes

Abstract

Background: In contrast to its role as poison, hydrogen sulfide (H2S) is recently considered as a gaso-transmitter which mediates important physiologic functions in humans. Evidence is accumulating to demonstrate that inhibitors of H2S production or therapeutic H2S donor compounds exert significant effects in various experimental models. Carbonic anhydrases (CA) are a group of zinc-containing metalloenzymes that catalyse the reversible hydration of carbon dioxide. CAs activity in erythrocytes (CAI and CAII) has recently been observed to be associated with various pathological conditions especially in diabetes mellitus, hypertension and lipid disorders. Alteration of this enzyme activity has been reported by the effect of advanced glycation end products methylglyoxal and reduced glutathione.   

Aims and Objectives: As H2S, being a mediator of many physiological functions and synthesized in vivo, may affect functions of many intracellular proteins like carbonic anhydrase, the objective of this study is to find out if there is any change in the carbonic anhydrase activity under the effect of H2S- donor NaHS in dose dependant manner using RBC model in vitro.

Materials and Methods: Blood sample was collected from forty (40) numbers of healthy volunteers of 18-40 years of in heparin containing vials and packed cells were prepared immediately by centrifugation  The packed erythrocytes were washed three times with normal saline and  diluted (1:10) with the normal saline. One ml each of diluted packed cells was taken in eight test tubes. Serial dilutions of NaHS (1to 250 µMol/L) was added to all the test tubes except for the first test tube where only normal saline was added and   incubated at room temperature for one hour. Haemolysates was prepared from the erythrocytes with equal volume of distilled water in each tube and the CA activity was determined in the haemolysates using standardized method.

Results: There is significant increase of CA activity in dose dependent manner under the effect of NaHS and also compared to the activity of hemolysate prepared without NaHS.  

Conclusions:Our study for the first time demonstrated that the Carbonic Anhydrase activity of erythrocytes is significantly increases by the effect of NaHS and this study reveals some important biological role of H2S and carbonic anhydrase.

Asian Journal of Medical Sciences Vol. 7(3) 2016 23-27

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Author Biography

Arun Kumar, Professor, Department of Biochemistry, Shri Shankaracharya Institute of Medical Sciences, Bhilai, Chhattisgarh

Biochemistry, Professor

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Published

2016-01-06

How to Cite

Bhakta, A., Bandyopadhyay, M., Dasgupta, S., Sen, S., Kumar, A., & Biswas, U. K. (2016). Effect of NaHS on carbonic anhydrase activity of human erythrocyte. Asian Journal of Medical Sciences, 7(3), 23–27. https://doi.org/10.3126/ajms.v7i3.14047

Issue

Vol. 7 No. 3 (2016)

Section

Original Articles

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