Evaluation of anti-inflammatory activity of ethanolic extract of Derris brevipes (benth.) Baker leaves

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DOI:

https://doi.org/10.3126/ajms.v14i10.55697

Keywords:

Musculoskeletal and joint diseases; Arthritis; Rheumatoid arthritis; Collagen induced arthritis

Abstract

Background: Ethnopharmacological relevance: Derris brevipes (benth.) Baker is widely used as an anti-arthritic agent in traditional medicine.

Aims and Objectives: This study evaluated the anti-inflammatory activity of ethanolic extract of D. brevipes (EEDB) leaves, a member of the Leguminosae family in vitro and ex vivo.

Materials and Methods: The EEDB leaves of was screened for anti-inflammatory activities by protein denaturation analysis and proteinase inhibition analysis. Prednisolone was used as the standard. EEDB (at doses of 300 mg/kg and 600 mg/kg) was administered through the oral route. Ex vivo analysis was performed by estimating the expression of tumor necrosis factor-alpha (TNF-α) in collagen (chicken sternal collagen)-induced arthritis (CIA) in Sprague–Dawley rats using the reverse transcription polymerase chain reaction method. The data were analyzed using one-way analysis of variance, followed by Dunnet’s multiple comparison tests.

Results: Prednisolone (100 mcg/mL) and EEDB (600 mcg/mL) showed percentage inhibition of 80.32% and 80.04%, respectively, in protein denaturation assay. Similarly, prednisolone (600 mcg/mL) and EEDB (600 mcg/mL) showed 66.03% and 56.69% inhibition in the proteinase activity. Synovial tissue TNF-α levels showed a significant decrease in EEDB-treated groups and prednisolone-treated group compared to CIA control rats.

Conclusion: From this study, it was concluded that the D. brevipes leaf extract possesses anti-inflammatory activity, which explains its beneficial role in rheumatoid arthritis.

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Author Biographies

Nesni M Makkar, Associate Professor, Department of Pharmacology, Al-Azhar College of Pharmacy, Thodupuzha, Kerala, India

 

 

Veena R, Professor, Department of Pharmaceutical Sciences, Center for Professional and Advanced Studies, Kottayam, Kerala, India

 

 

Sancy Mary Sam, Associate Professor, Department of Pharmacology, Al-Azhar College of Pharmacy, Thodupuzha, Kerala, India

 

 

Jeena Ann John, Assistant Professor, Department of Pharmacology, Al-Azhar College of Pharmacy, Thodupuzha, Kerala, India

 

 

Sanitha Kuriachan, Associate Professor, Department of Pharmacology, Amrita School of Medicine, Amrita Institute of Medical Sciences, Amrita Vishwa Vidyapeetham, Kerala, India

 

 

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Published

2023-10-02

How to Cite

Nesni M Makkar, Veena R, Sancy Mary Sam, Jeena Ann John, & Kuriachan, S. (2023). Evaluation of anti-inflammatory activity of ethanolic extract of Derris brevipes (benth.) Baker leaves. Asian Journal of Medical Sciences, 14(10), 67–72. https://doi.org/10.3126/ajms.v14i10.55697

Issue

Vol. 14 No. 10 (2023)

Section

Original Articles

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