TY - JOUR AU - Rajkumar, Kalyan AU - Radera, Shruti AU - Agarwal, Jyotsna AU - Singh, Mastan PY - 2015/11/05 Y2 - 2024/03/29 TI - Superbugs causing infections at a tertiary care hospital and the return of pre-antibiotic era! JF - Asian Journal of Medical Sciences JA - Asian J Med Sci VL - 7 IS - 2 SE - Original Articles DO - 10.3126/ajms.v7i2.12750 UR - https://www.nepjol.info/index.php/AJMS/article/view/12750 SP - 76-79 AB - <p><strong>Aims and Objectives: </strong>Increase in the incidence of <em>Escherichia coli </em>and <em>Klebsiella pneumoniae </em>carrying New Delhi metallo beta lactamase-1 (NDM-1) gene are called superbugs is of great concern as presence of <em>bla</em>NDM-1 gene makes <em>E.coli </em>and <em>K.pneumoniae </em>highly resistant to most of currently available antibiotics. This study was planned to observe the burden of bla<sub>NDM-1</sub> gene carrying <em>E. coli </em>and <em>K.pneumoniae </em>at a tertiary care hospital in northern India. <strong></strong></p><p><strong>Materials and Methods: </strong>A total of 1709 <em>E. coli </em>and 327 <em>K. pneumonia </em>nonrepitive isolates were taken from various clinical samples received in a tertiary care hospital in northern India Lucknow during the period from May 2012 to April 2013. Carbapenemase production was phenotypically detected in all the carbapenem resistant isolates by modified Hodge test. Metallo-β-lactamase production was detected by using meropenem and imipenem discs with and without EDTA and <em>bla</em><sub>NDM-1</sub> gene was detected by polymerase chain reaction. <strong></strong></p><p><strong>Results: </strong>Over all metallo β- lactamase production was found in 82% and 88.89% of carbapenem resistant <em>E.coli </em>and <em>K. pneumonia </em>respectively. Out of 366 carbapenem resistant isolates, 102 were found positive for <em>bla</em><sub>NDM-1</sub> gene out of which 89 were <em>E.coli </em>and 13 were <em>K. pneumoniae</em>. <strong></strong></p><p><strong>Conclusions: </strong>With limited treatment options left for this crisis situation like the pre-antibiotic era; it is an alarm for rational antibiotic therapy usage and intensive education programs.</p><p>Asian Journal of Medical Sciences Vol.7(2) 2015 76-79</p> ER -