<i>In vitro</i> culture of <i>Piper longum</i> Linn.

  • K. M. Rajkarnikar National Herbarium and Plant Laboratory, Godawari
  • G. D. Bhatta National Herbarium and Plant Laboratory, Godawari
Keywords: vitro culture, Nepal

Abstract

In vitro clonal propagation of Piper longum is attempted for rapid multiplication using shoot tip explants. The explants regenerated micro shoots along with few callus and embryoids on the Murashige and Skoog (1962) supplemented with 1mg/l BAP and 0.1mg/l NAA. The NAA concentration of the same medium was lowered to 0.01 mg/l and added 10% coconut milk for subculture. For rooting, these regenerated microshoots were transferred to non-sterile sand, after acclimatization. They initiated roots within 15 to 20 days and transferred to polybags for further growth and for field transfer.

Bulletin of the Department of Plant Resources Vol 30, 2008 pp 76-79

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Abstract
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Author Biographies

K. M. Rajkarnikar, National Herbarium and Plant Laboratory, Godawari
National Herbarium and Plant Laboratory, Godawari
G. D. Bhatta, National Herbarium and Plant Laboratory, Godawari
National Herbarium and Plant Laboratory, Godawari
Published
2008-12-23
How to Cite
Rajkarnikar, K., & Bhatta, G. (2008). <i>In vitro</i> culture of <i>Piper longum</i&gt; Linn. Bulletin of the Department of Plant Resources, 30, 76-79. Retrieved from https://www.nepjol.info/index.php/BDPR/article/view/1530
Section
Articles