Status of antioxidant defense system for detoxification of arsenic in Brassica juncea (L.)

Authors

  • Mohd. Kafeel Ahmed Ansari Cyanobactrial Biotechnology Laboratory, Department of Biosciences, Faculty of Natural Sciences Jamia Millia Islamia (A Central University), New Delhi, 110025, India. Molecular Ecology Laboratory, Department of Botany, Faculty of Science, Hamdard University, New Delhi, 110062, India Environmental Contaminants Group, Mawson Institute, University of South Australia
  • Munir Hussain Zia Environmental Chemistry Laboratory, British Geological Survey, Keyworth
  • Altaf Ahmad Nanobiotechnology Laboratory, Department of Botany, Faculty of Life Sciences, Aligarh Muslim University, Aligarh
  • Ibrahim Mohammed Aref Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451
  • Tasneem Fatma Cyanobactrial Biotechnology Laboratory, Department of Biosciences, Faculty of Natural Sciences Jamia Millia Islamia (A Central University), New Delhi, 110025
  • Muhammad Iqbal Molecular Ecology Laboratory, Department of Botany, Faculty of Science, Hamdard University, Hamdard Nagar, New Delhi, 110062
  • Gary Owens Environmental Contaminants Group, Mawson Institute, University of South Australia, Mawson Lakes, SA 5095

DOI:

https://doi.org/10.3126/eco.v22i0.15466

Keywords:

Antioxidative enzymes, AsA-GSH pathway, chloroplast pigments, oxidative stress, seed germination

Abstract

The content of arsenic (As), a naturally occurring toxic element found in soils worldwide, has gone substantially high in agricultural soils due to various anthropogenic activities. The responses of seed germination, seedling growth, photosynthetic pigments and the components of the ascorbateglutathione (AsA-GSH) pathway were analyzed in Indian mustard [Brassica juncea (L.) Czern.] cultivar Pusa Jai Kisan, treated with 0, 5, 10, 25 and 50 μM As concentrations in a hydroponic system. While a significant increment in lipid peroxidation and H2O2 generation was observed at higher concentrations after 2 and 5 days of stress imposition. Significant increases were also observed in superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) activities under the influence of 25 and 50 μM As concentrations after 2 and 5 d stress impositions. There was a steep increase in the ascorbic acid (AsA) content between 2 and 5 d after As treatment, while glutathione (GSH) content increased significantly after 5-d-exposure. Accumulation of As was more in shoot than in root. This study clearly demonstrated that high concentration (50 μM) of As inhibited growth and caused oxidative stress and that the AsA-GSH pathway has an important role in cellular defense against As in Brassica juncea (L.).

ECOPRINT 22: 7-19, 2015

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Published

2016-09-22

How to Cite

Ansari, M. K. A., Zia, M. H., Ahmad, A., Aref, I. M., Fatma, T., Iqbal, M., & Owens, G. (2016). Status of antioxidant defense system for detoxification of arsenic in Brassica juncea (L.). Ecoprint: An International Journal of Ecology, 22, 7–19. https://doi.org/10.3126/eco.v22i0.15466

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Articles