ESTIMATING BACTERIAL POPULATION ON THE PHYLLOSPHERE BY SERIAL DILUTION PLATING AND LEAF IMPRINT METHODS

Authors

  • Ram Kailash P. Yadav Professor, Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu
  • Katerina Kakamanoli Laboratory of Agricultural Chemistry, School of Agriculture, Aristotle University, Thessaloniki
  • Despoina Vokou Department of Ecology, School of Biology, Aristotle University, Thessaloniki

DOI:

https://doi.org/10.3126/eco.v17i0.4105

Keywords:

Phyllosphere, Bacterial population, Serial dilution plating, Leaf imprint

Abstract

The plant phyllosphere constitute a habitat for variety of microorganisms; bacteria are the most common ones. In this study, we assessed the ability of serial dilution plating and leaf imprint methods to elucidate population abundance on the phyllosphere of eight plant species co-occurring in the same
area in mediterranean ecosystem (northern Greece).  The serial dilution plating method outweighed the leaf imprint method in determining the total epiphytic bacterial population and the estimates were significantly higher. However, the ability to detect presence of bacterial colony on leaf surface is higher for the leaf imprints, suggesting specific use of this method for practical purposes.

Key words:  Phyllosphere; Bacterial population; Serial dilution plating; Leaf imprint

DOI: 10.3126/eco.v17i0.4105

Ecoprint

An International Journal of Ecology

Vol. 17, 2010

Page: 47-52

Uploaded date: 29 December, 2010

 

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How to Cite

Yadav, R. K. P., Kakamanoli, K., & Vokou, D. (2011). ESTIMATING BACTERIAL POPULATION ON THE PHYLLOSPHERE BY SERIAL DILUTION PLATING AND LEAF IMPRINT METHODS. Ecoprint: An International Journal of Ecology, 17, 47–52. https://doi.org/10.3126/eco.v17i0.4105

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