@article{Sah_Regmi_Tamang_2017, title={Antibacterial Effects of Thuja Leaves Extract}, volume={5}, url={https://www.nepjol.info/index.php/IJASBT/article/view/17617}, DOI={10.3126/ijasbt.v5i2.17617}, abstractNote={<p>Various medicinal plants are common in use in Nepal for the treatment of different diseases. Nowadays, drug resistance has emerged as a major problem for various infections, in such case plants can be used as alternative for the production of new antimicrobial agents. <em>Thuja</em> (in Nepali: <strong><em>dhupi</em></strong>) is a small evergreen genus of the Cupressaceae family. This species is widely cultivated as a common ornamental plant in Nepal and India. This study examined the antibacterial activity of <em>Thuja</em> leaves extract on gram positive (<em>Staphylococcus aureus</em> and <em>Streptococcus</em> spp.) and gram-negative bacteria (<em>E. coli</em> and <em>Pseudomonas aeruginosa)</em>. <em>Thuja</em> leaves were collected from different localities of Dharan, and dried under shade for 10 days. They were then grinded using mechanical grinder. Leaf extract (oleoresin) was obtained by soxhlet extraction technique using mixture of Ethyl acetate, Ethanol and chloroform in the ratio 40:30:30 as the solvent. The antibacterial activity of <em>Thuja</em> oleoresin was tested using both Agar well diffusion as well as disc diffusion technique. Minimum Inhibitory Concentration(MIC) was  determined by agar well diffusion on MHA plates. <em>Thuja</em> oleoresin showed distinct antibacterial activity towards all four isolates on both agar well and disc diffusion methods. MIC for <em>Pseudomonas aeruginosa </em>and <em>Streptococcus</em> spp. was found to be 12.5 µl whereas for <em>E. coli </em>and <em>Staphylococcus aureus, MIC was</em> 25 µl. Thus from our results, it is concluded that <em>Thuja</em> leaves have antibacterial effects and can be a potential source for production of antibacterial drugs.</p><p><em>Int. J. Appl. Sci. Biotechnol. Vol 5(2): 256-260</em></p>}, number={2}, journal={International Journal of Applied Sciences and Biotechnology}, author={Sah, Shiv Nandan and Regmi, Sunil and Tamang, Man Kumar}, year={2017}, month={Jun.}, pages={256–260} }