@article{Shahi_Bhattarai_Poudel_Pradhan_Pradhananga_Awal_2018, title={Green Synthesis of Silver Nanoparticles Using Different Plant Materials and Their Antibacterial Activity}, volume={6}, url={https://www.nepjol.info/index.php/IJASBT/article/view/22112}, DOI={10.3126/ijasbt.v6i4.22112}, abstractNote={<p>The green route of metal nanoparticles synthesis has received significant attention in recent years due to it’s cost-effective, non-toxic and eco-friendly nature in comparison to other physical and chemical methods. This study reports on the synthesis of silver nanoparticles (Ag-NPs) from bio-reduction of 1mM aqueous silver nitrate (AgNO<sub>3</sub>) by extracts prepared from three different plants namely, <em>Brassica oleracea</em> L<em>. var. italica Plenck </em>(Broccoli), <em>Capsicum annuum </em>L. (Chili) and <em>Parthenium hysterophorus </em>L. (Carrot grass). The synthesized Ag-NPs were characterized using UV- visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Ag-NPs synthesized showed the surface plasmon resonance with the appearance of absorption peaks around the range of 410-430 nm. The possible biomolecules involved in the reduction and the stabilization of synthesized Ag-NPs were found to be alcoholic, phenolic, amine and carbonyl groups. SEM study revealed that Ag-NPs were spherical in shape with varied size about 10-40 nm. Besides, the analysis of antioxidant and antibacterial activities of Ag-NPs was carried out. The Ag-NPs synthesized using <em>B. oleracea</em> extract showed the higher antioxidant activity than Ag-NPs synthesized from both <em>C. annuum</em> & <em>P. hysterophorus</em> extracts. Ag-NPs exhibited good antibacterial activity against both Gram-negative (<em>Escherichia coli)</em> and Gram-positive (<em>Staphylococcus aureus) </em>bacteria<em>.</em> The higher antibacterial activity was shown by Ag-NPs synthesized from <em>P. hysterophorus</em> extract in comparison to Ag-NPs synthesized from both <em>C. annuum </em>& <em>B. oleracea</em> extracts. Hence, it can be concluded that Ag-NPs synthesized following the green route could be the source for potential antioxidant and antibacterial agents.</p> <p><em>Int. J. Appl. Sci. Biotechnol. Vol 6(4): 294-301</em></p>}, number={4}, journal={International Journal of Applied Sciences and Biotechnology}, author={Shahi, Dipesh and Bhattarai, Elisha and Poudel, Milan and Pradhan, Prarthana and Pradhananga, Raja Ram and Awal, Suvash Chandra}, year={2018}, month={Dec.}, pages={294–301} }