@article{Sankhi_Sapkota_Regmi_2019, title={Effect of Age and Frequency of Collection on Quality of Jersey Bulls Semen at National Livestock Breeding Center (NLBC), Nepal}, volume={7}, url={https://www.nepjol.info/index.php/IJASBT/article/view/23312}, DOI={10.3126/ijasbt.v7i1.23312}, abstractNote={<p><strong><em>Objective</em></strong>: To determine the effect of age and frequency of collection on quality parameters of Jersey bull semen at National Livestock Breeding Center (NLBC), Pokhara.</p> <p><strong><em>Materials and Methodology</em></strong><strong>:</strong> Nine Jersey bulls were selected randomly from NLBC. To obtain the effect of age, six bulls of three different age interval (3-4, 5-7 and 8-9 years) were selected randomly. Remaining three bulls were used to determine the effect of frequency of collection on semen quality. Studies were conducted for three months (Nov/Dec to Dec/Jan, 2016). Semen quality was analyzed using CASA system and SMILE software. ANOVA and paired t- tests were applied to find out level of significance. All the statistical analyses were done using SPSS 17.0</p> <p><strong><em>Results</em></strong>: Sperm concentration, post- thaw motility, live sperm, and abnormal sperm were significantly affected by the age of the bulls. Semen quality was found superior in the bulls belonging to 5-7 years of age intervals. Significantly higher value for semen volume, sperm concentration and post- thaw motility was obtained on second collection (P<0.05). However, volume initial motility, pre-filling motility, live sperm and abnormal sperm percentage did not differ significantly among different age intervals and between the frequencies of collection.</p> <p><strong><em>Conclusion</em></strong>: Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentages were higher in 5-7 years old bulls and in second collection of semen.</p> <p>Int. J. Appl. Sci. Biotechnol. Vol 7(1): 88-95</p>}, number={1}, journal={International Journal of Applied Sciences and Biotechnology}, author={Sankhi, S. and Sapkota, K.R. and Regmi, B.}, year={2019}, month={Mar.}, pages={88–95} }