TY - JOUR AU - Kalra, Shipra AU - Midha, Kanav AU - Monika, - AU - Kaur, Manpreet PY - 2016/03/31 Y2 - 2024/03/29 TI - Studies on Anti-Cancer Activity of Lysyl Oxidase from Trichoderma Viride MTCC 167 JF - International Journal of Applied Sciences and Biotechnology JA - Int J Appl Sci Biotechnol VL - 4 IS - 1 SE - Research Articles: Biological Sciences DO - 10.3126/ijasbt.v4i1.14378 UR - https://www.nepjol.info/index.php/IJASBT/article/view/14378 SP - 57-63 AB - <p>Lysyl oxidase produces H<sub>2</sub>O<sub>2 </sub>i.e. ROS by acting on L-Lysyine. In the present study, ROS thus produced has been used by <em>in vitro </em>cytotoxicity assay on ovarian cancer cells thereby achieving 250 percent inhibition. Lysyl oxidase activity was determined spectrophotometrically by Dintrophenylhydrazine (DNPH) reagent. For isolation of lysyl oxidase produced by <em>Trichodermaviride</em>, acetone precipitation and ammonium sulphate precipitation was carried out. The effect of temperature on lysyl oxidase production was determined by incubating the media with 1.5 % inoculum at different temperature ranging from  10 , 20, 30,40,50,60,70 , 80 <sup>0</sup>C for 72 hr . Anti-tumor properties of Lysyl oxidase was checked using Rhodamine assay and NBT Assay.  Comparative results for Acetone and Ammonium Sulphate precipitation showed that Enzyme activity(U/ml)  of acetone precipitation is 124.6 and 144.3 IU/ml and Protein Content is 1.8 and 2.2 mg/ml with the specific activity 68.4IU/mg and 64.1IU/mg . The optimum enzyme production was found to be at pH 8 and optimum temperature for lysyl oxidase production was 50<sup>0</sup>C with maximum enzyme activity of 0.38 (U/ml). 7<sup>th</sup> fraction contained highest enzyme activity so the retention time of lysyl oxidase was found to be 7 min. The protein content was also calculated by using Bradford method and it was highest in the 1<sup>st</sup> fraction and in 6<sup>th</sup>, 7<sup>th</sup> and 8<sup>th </sup>fractions. The specific activity has been improved from 75.1 IU/mg to 86.5 IU/mg. 10 units of lysyl oxidase inhibits 3x10<sup>4 </sup>cells in each well to 82.5% and inhibition achieved at same cell count at 200 units which was 250% as observed with NBT and Rhodamine assay.</p><p>Int J Appl Sci Biotechnol, Vol 4(1): 57-63</p> ER -