TY - JOUR AU - Rajendiran, K AU - Vidya, S AU - Arulmozhi, D PY - 2016/03/31 Y2 - 2024/03/28 TI - In Vitro Callus Proliferation from Leaf Explants of Green Gram after In Situ UV-B Exposure JF - International Journal of Applied Sciences and Biotechnology JA - Int J Appl Sci Biotechnol VL - 4 IS - 1 SE - Research Articles: Biological Sciences DO - 10.3126/ijasbt.v4i1.14719 UR - https://www.nepjol.info/index.php/IJASBT/article/view/14719 SP - 82-91 AB - <p>Callus induction was tried with leaf explants (third leaf from top of canopy) harvested from <em>in situ</em> control and supplementary UV-B irradiated (UV-B = 2 hours daily @ 12.2 kJ m<sup>-2</sup> d<sup>-1</sup>; ambient = 10 kJ m<sup>-2</sup> d<sup>-1</sup>) three varieties of green gram<em> viz.</em> CO-8, NVL-585 and VAMBAN-2 to study their viability for germplam conservation. Callus induction occurred both in control and UV-B stressed NVL-585 leaf explants. VAMBAN-2 both in unstressed and UV-B stressed conditions did not initiate callus. Only control leaf explants from CO-8 proliferated callus. Callus of UV-B irradiated NVL-585 weighed less (51.28 %) than control. Parenchyma cells were smaller in callus inducted from <em>in situ</em> UV-B exposed NVL-585 leaf explants. The leaf explants from UV-B stressed NVL-585 varieties of green gram responded to <em>in vitro</em> callus proliferation making them fit for germplasm conservation for cultivating in UV-B elevated environment.</p><p>Int J ApplSci Biotechnol, Vol 4(1): 82-91</p> ER -