TY - JOUR AU - Vidya, P.V. AU - Chitra, K.C. PY - 2017/06/29 Y2 - 2024/03/29 TI - Induction of Cytotoxicity by Selected Nanoparticles in Chinese Hamster Ovary-K1 Cells JF - International Journal of Applied Sciences and Biotechnology JA - Int J Appl Sci Biotechnol VL - 5 IS - 2 SE - Research Articles: Biological Sciences DO - 10.3126/ijasbt.v5i2.17619 UR - https://www.nepjol.info/index.php/IJASBT/article/view/17619 SP - 203-207 AB - <p>The aim of the present study is to analyze the cytotoxicity of selected nanoparticles on Chinese Hamster Ovary-K1 (CHO-K1) cells using methyl tetrazolium (MTT) assay and lactate dehydrogenase (LDH) release assay. Four different metal oxide nanoparticles namely silicon dioxide (SiO<sub>2</sub>-NPs, 1 nm), aluminium oxide (Al<sub>2</sub>O<sub>3</sub>-NPs, 16.7 nm), titanium dioxide (TiO<sub>2</sub>-NPs, 11.4 nm) and iron oxide (Fe<sub>3</sub>O<sub>4</sub>-NPs, 15.65 nm) were exposed to CHO-K1 cells at 25, 50, 75 and 100 µg/ml concentrations for 24 h maintaining the control group. The percentage of cell viability using methyl tetrazolium (MTT) assay showed significant reduction in cell viability from 63.82 to 31.19% in SiO<sub>2</sub>-NPs, 96.68 to 34.14% in Al<sub>2</sub>O<sub>3</sub>-NPs, 65.69 to 14.32% in TiO<sub>2</sub>-NPs and 120.69 to 59.86% in Fe<sub>3</sub>O<sub>4</sub>-NPs when compared with the untreated cells. Assessment of cytotoxicity by using lactate dehydrogenase (LDH) release assay revealed that Al<sub>2</sub>O<sub>3</sub>–NPs showed more cytotoxicity followed by Fe<sub>3</sub>O<sub>4</sub>-NPs, TiO<sub>2</sub>-NPs and SiO<sub>2</sub>-NPs in concentration-dependent manner. Therefore, it is reasonable to conclude that size and the composition of the nanoparticles could contribute to the relative cytotoxicity in CHO cells.</p><p><em>Int. J. Appl. Sci. Biotechnol. Vol 5(2): 203-207</em></p> ER -