TY - JOUR AU - Shahi, Dipesh AU - Sapkota, Rajiv PY - 2018/12/27 Y2 - 2024/03/28 TI - A Comparative Study on Dye Degradation by Leaf and Root Extracts of Parthenium hysterophorus L JF - International Journal of Applied Sciences and Biotechnology JA - Int J Appl Sci Biotechnol VL - 6 IS - 4 SE - Research Articles: Biological Sciences DO - 10.3126/ijasbt.v6i4.22110 UR - https://www.nepjol.info/index.php/IJASBT/article/view/22110 SP - 327-331 AB - <p>The use of different dyes and pigments is increasing with the increase in industrialization leading to the high production of effluent. The effluent contaminated with dyes and dye-stuff has harmful effects on public health and the environment. Thus, the treatment of effluent is essential. Biological approaches are gaining much interest due to their cost-effective and eco-friendly nature over various physicochemical methods for the treatment of dye-contaminated wastewater. This study highlights on the biodegradation of congo red and malachite green by using leaf and root extracts of <em>Parthenium hysterophorus. </em>The extract and the dye were mixed in the ratio of 1:2 and incubated at 40ÂșC for 90 minutes. Decolorization assay was performed using UV visible spectrophotometer which indicated that decolorization was due to degradation of dyes into non-colored metabolites. The leaves extract exhibited higher decolorizing activity than roots extract. The maximum decolorization for leaves extract was 55.8% (congo red) and 51.6% (malachite green). Furthermore, phytotoxicity test was carried out to determine the effect of dyes and their degradation metabolites on seed germination and seedling growth of chickpea (<em>Cicer arietinum </em>L). The germination percentage and seedling growth were more in degradation metabolites than untreated dyes, indicating less toxic nature of degradation metabolites. Hence, it can be inferred that <em>P. hysterophorus </em>extracts can be used to treat dye wastewater and treated wastewater can be used for irrigation.</p><p>Int. J. Appl. Sci. Biotechnol. Vol 6(4): 327-331</p> ER -