TY - JOUR AU - Joshi, Sushika AU - Karna, Anish Kumar PY - 2013/09/25 Y2 - 2024/03/29 TI - Analysis of Phytoconstiuents and Cytotoxic Activities of Different Parts of Ocimum sanctum JF - International Journal of Applied Sciences and Biotechnology JA - Int J Appl Sci Biotechnol VL - 1 IS - 3 SE - Research Articles: Biological Sciences DO - 10.3126/ijasbt.v1i3.8609 UR - https://www.nepjol.info/index.php/IJASBT/article/view/8609 SP - 137-144 AB - <p>Phytochemical screening of extracts of<strong> </strong><em>Ocimum Sanctum </em>revealed the presence of fatty acids, steroids, triterpenoid, phenolic compound, flavonoid, glycoside, quinines, and carotenoids. &beta;-sitosterol and Ursolic acid were isolated from leaves of the plant by using column chromatography and&nbsp; identification&nbsp; of these compounds were performed with the help of melting points, Co-TLC&nbsp; and spectroscopic techniques such as IR, <sup>1</sup>HNMR and <sup>13</sup> CMR. GC-MS of hexane fraction showed presence of pentanal, sotolone, hexane-3-one, hexane-2-ol, caryphyllene, benzene 1,2 di-carbolylic acids, isoeugenol, cavracrol and eugenol compounds. The brine shrimp bio assay showed &nbsp;LC<sub>50</sub> value 213.7, &nbsp;97.72 and 21.87 for hexane, ethyl acetate and methanol extracts of leaves respectively, where as LC<sub>50</sub> value 169.84, 141.25 and 151.35 for hexane, ethyl acetate and methanol &nbsp;extracts of stem respectively. All extracts are pharmacologically active.</p><p>DOI: <a href="http://dx.doi.org/10.3126/ijasbt.v1i3.8609">http://dx.doi.org/10.3126/ijasbt.v1i3.8609</a></p> <p>Int J Appl Sci Biotechnol, Vol. 1(3) 2013 : 137-144</p> ER -