Solid State Fermentation of <i>Aspergillus oryzae</i> for Glucoamylase Production on Agro residues

  • Vasudeo Zambare R&D Laboratory, Sequence Biotech Pvt. Ltd., Wadivarhe, Tal- Igatpuri, Dist- Nashik
Keywords: Aspergillus oryzae

Abstract

Glucoamylase is a well recognized amylolytic enzyme used in food industry, which is generally produced by Aspergillus genus under solid-state fermentation (SSF). This study presents production of glucoamylase by Aspergillus oryzae on the solid surface of rice husk, wheat bran, rice bran, cotton seed powder, corn steep solids, bagasse powder, coconut oil cake, and groundnut oil cake as substrates. Optimization of the SSF media and parameters resulted in a 24% increase in the glucoamylase activity. Optimum glucoamylase production (1986 μmoles of glucose produced per minute per gram of dry fermented substrate) was observed on wheat bran supplemented with 1%, (w/w) starch, 0.25%, (w/w) urea at pH 6, 100%, (v/w) initial moisture and 30°C after incubation 120 hrs. Therefore, A. oryzae can be useful in bioprocessing application for saccharification of agro-residues.

Keywords: Glucoamylase, Aspergillus oryzae, solid state fermentation, agro residues

DOI: 10.3126/ijls.v4i0.2892

International Journal of Life Sciences Vol.4 2010 pp.16-25

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Author Biography

Vasudeo Zambare, R&D Laboratory, Sequence Biotech Pvt. Ltd., Wadivarhe, Tal- Igatpuri, Dist- Nashik

Present Address: South Dakota School of Mines and Technology, 501, E. Saint Joseph Street, Rapid City 57701, SD, USA. Phone: +1-605-394-3384.

Email: vasuzambare@gmail.com; Vasudeo.Zambare@sdsmt.edu

Published
2010-03-15
How to Cite
Zambare, V. (2010). Solid State Fermentation of <i>Aspergillus oryzae</i&gt; for Glucoamylase Production on Agro residues. International Journal of Life Sciences, 4, 16-25. https://doi.org/10.3126/ijls.v4i0.2892
Section
Research Articles