Prevalence of carbapenem resistant bacterial strains isolated from different clinical samples: study from a tertiary care hospital in Kathmandu, Nepal

Authors

  • Sitesh Karn Post graduate student (M.Sc.), Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu
  • Narayan Dutt Pant Department of Microbiology, Grande International Hospital, Dhapasi, Kathmandu
  • Sanjeev Neupane Post graduate student (M.Sc.), Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu
  • Saroj Khatiwada Lecturer, Department of biochemistry, Modern Technical College, Lalitpur, Kathmandu
  • Shaila Basnyat Lecturer, Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu
  • Basudha Shrestha Senior Microbiologist, Kathmandu Model Hospital, Kathmandu

DOI:

https://doi.org/10.3126/jbs.v3i1.16846

Keywords:

Multidrug resistance, carbapenemase, metallo-β-lactamase, Klebsiella pneumoniae carbapenemase, AmpC-β-lactamase, Nepal

Abstract

Background Carbapenems are considered as drugs of choice for the treatment of the infections caused by drug resistant bacteria. However, in the recent years the prevalence of carbapenem resistant gram negative bacteria has increased significantly. The main objective of this study was to determine the prevalence of carbapenemase producing gram negative bacteria among all the clinical isolates.

Material and methods A total of 3246 non-repeated, different clinical specimens from patients attending Kathmandu Model Hospital, from July 2013 to January 2014 were cultured and the gram negative bacterial isolates obtained were subjected to identification with the help of colony morphology, Gram’s stain and conventional biochemical tests. Kirby-Bauer disk diffusion technique was used to perform antimicrobial susceptibility testing. Phenotypic confirmation of carbapenemase and AmpC beta-lactamase production was done by combined disc method.

Results 890 samples showed the growth of bacterial pathogens. Out of total 769 gram negative bacteria, 57 were found to be carbapenem resistant. Of which, highest number (47) of the isolates were found to be metallo-β lactamase (MBL) producers. Six bacterial isolates produced both (Klebsiella pneumoniae carbapenemase) KPC and MBL, whereas only one isolate was found to be positive for both MBL and AmpC. Three bacterial strains showed carbapenem resistance due to over production of AmpC β-lactamase.

Conclusion Among carbapenem resistant gram negative bacteria, MBL was present as the major enzyme responsible for resisting carbapenem antibiotics.

Downloads

Download data is not yet available.
Abstract
2276
PDF
1833

Downloads

Published

2017-02-27

How to Cite

Karn, S., Pant, N. D., Neupane, S., Khatiwada, S., Basnyat, S., & Shrestha, B. (2017). Prevalence of carbapenem resistant bacterial strains isolated from different clinical samples: study from a tertiary care hospital in Kathmandu, Nepal. Journal of Biomedical Sciences, 3(1), 11–15. https://doi.org/10.3126/jbs.v3i1.16846

Issue

Section

Original Articles