TY - JOUR AU - Marla, Vinay AU - Baral, Dharanidhar AU - Pokharel, Prabhat Ranjan AU - Shrestha, Ashish AU - Gyawali, Rajesh AU - Giri, Jamal PY - 2019/10/01 Y2 - 2024/03/28 TI - Cellular Alteration of the Oral Mucosa due to Orthodontic Appliances and Tobacco Smoking: A Cytomorphometric Study JF - Journal of College of Medical Sciences-Nepal JA - J Coll Med Sci-Nepal VL - 15 IS - 3 SE - Original Articles DO - 10.3126/jcmsn.v15i3.22620 UR - https://www.nepjol.info/index.php/JCMSN/article/view/22620 SP - 206-210 AB - <p><strong>Introduction</strong>: Fixed orthodontic treatment causes chronic low grade irritation on the oral mucosa. Tobacco smoking is considered to be a risk factor in the development of oral cancer. This study involves a cytomorphometric analysis on the buccal mucosal cells in patients undergoing fixed orthodontic treatment who are tobacco smokers.</p><p><strong>Methods</strong>: Exfoliated buccal mucosal cells were obtained from orthodontic patients (smokers &amp; non-smokers) as well as smokers and non-smokers who are not undergoing any orthodontic treatment. Smears were stained with rapid PAP stain and digital images of 50 cells were analysed using the Image J software.</p><p><strong>Results:&nbsp;</strong>Statistical analysis revealed that there were significant increase in the values of nuclear and cell diameter between non-smokers undergoing orthodontic treatment and smokers (p&lt;0.001 &amp; p=0.014 respectively), and between non-smokers undergoing orthodontic treatment and smokers undergoing orthodontic treatment (p&lt;0.001 &amp; p=0.015 respectively). Also, nuclear area and cell area showed significant differences between many of the study groups.</p><p><strong>Conclusion</strong>: It can be concluded that there are nuclear and cellular alterations in patients undergoing fixed orthodontic treatment who are smokers</p> ER -