TY - JOUR AU - Jha, Brajesh Kumar AU - Adhikari, Neetu AU - Rajkumari, Sanjhana PY - 2021/09/30 Y2 - 2024/03/29 TI - Isolation, Identification and Antibiotic Susceptibility Patterns of Listeria Monocytogens from Pregnant Women JF - Journal of College of Medical Sciences-Nepal JA - J Coll Med Sci-Nepal VL - 17 IS - 3 SE - Original Articles DO - 10.3126/jcmsn.v17i3.37062 UR - https://www.nepjol.info/index.php/JCMSN/article/view/37062 SP - 227-233 AB - <p><strong>Introduction</strong><br />The present study is made to determine the prevalence rate, isolation, identification and antibiotic<br />susceptibility pattern of L. monocytogens isolated from pregnant women. This research not only<br />guides to the clinician for treatment but also help laboratory worker to monitor susceptibility<br />pattern among the Listeria species.</p><p><strong>Methods</strong><br />The samples were collected from Gynecology department from January 2017 to December 2019<br />and processed in the Department of Microbiology at College of Medical Sciences, Bharatpur Nepal.<br />Two hundred thirty four antenatal mothers having age between 17 and 39 years of gestation period<br />between 7th weeks and 36th weeks patient with a flue like sign and symptoms were screened for<br />L. monocytogens. Bacterial isolation identification and antibiotic sensitivity tests were done as per<br />standard microbiological guidelines.</p><p><strong>Results</strong><br />In this present research, the prevalence of L. monocytogens was found 39/234 (16.7%), where high rate of Listeria infection was found 21(53.1%) among the patients of age between 25 and 32 who were mostly from urban area. We found eating meat 38(97.4%) and fish 39(100.0%), non-pasteurized simple boiled milk 39(100.0%) and vegetables like salad eater 32(82.1%) has highest rate of listeriosis. The results showed most of the listeriosis were occurring in a 3rd trimester 17(43.6%) compared with the 2nd and 1st trimester. Overall, the antibiotics resistance pattern found low in L. monocytogens. This investigation also showed less resistance in erythromycin 9(23.1%), ciprofloxacin 8(20.5%), chloramphenicol 16(41.1%). The increased resistance pattern among Listeria species were found in penicillin 31(79.5%), cotrimoxazole 20(51.3%), amoxicillin 21(55.1%) and vancomycin 29(74.4%).</p><p><strong>Conclusions</strong><br />It is very important to consider listeriosis in a clinical practice now a day. Maternal Listeriosis should<br />be considered in all age group of pregnant women. Regular surveillance of antibiotic susceptibility test is very much important for clinician to select best medicine to treat different cases of listeriosis.</p> ER -