TY - JOUR AU - Sharma, Khaga Raj AU - Adhikari, Achyut AU - Choudhary, M. Iqbal AU - Awale, Suresh AU - Kalauni, Surya Kant PY - 2015/11/25 Y2 - 2024/03/28 TI - Bioassay Guided Isolation of Free Radical Scavenging Agent from the Bark of Bridelia retusa JF - Journal of Institute of Science and Technology JA - J. Inst. Sci. Tech. VL - 20 IS - 1 SE - Research Articles DO - 10.3126/jist.v20i1.13916 UR - https://www.nepjol.info/index.php/JIST/article/view/13916 SP - 97-101 AB - <p>Bioassay guided fractionation of ethyl acetate soluble fraction of methanolic extract of <em>Bridelia retusa </em>yielded tambulin (<strong>1</strong>), <em>beta</em>-sitosterol (<strong>2</strong>), and <em>beta</em>-sitosterol glucoside (<strong>3</strong>), and their structures were elucidated using modern spectroscopic techniques. Compounds <strong>1</strong>-<strong>3 </strong>were evaluated for their antioxidant activity and compound <strong>1 </strong>was found to be potent antioxidant. The DPPH assay showed that the compound <strong>1 </strong>has potent antioxidant activity with IC<sub>50</sub> 166.15±1.92 SEM [muM] and the radical scavenging activity was found to be 86.03% where as the standard butylated hydroxy toluene (BHT) has IC<sub>50</sub> value 128.83±2.1 SEM [muM]. The radical scavenging activity of standard butylated hydroxy toluene was about 86% .The total phenolic content in ethyl acetate soluble fraction was found to be 147.20±1.5 mg GAE/gm and the total flavonoid content was found to be 16.64±0.00 mg QE/gm.</p><p>Journal of Institute of Science and Technology, 2015, <strong>20(1)</strong>: 97-101</p> ER -