Antibiotic Susceptibility Patterns and Extended Spectrum Beta-Lactamase (ESBL) production in Enterobactericeae Isolated from Stool Samples of HIV and AIDS Patients in Ibadan, Nigeria

Authors

  • Olutayo Israel Falodun University of Ibadan, Nigeria
  • Olayemi Ajayi University of Ibadan, Nigeria
  • Adewuyi Ebenezer Ademola Caleb University, Imota, Lagos, Nigeria
  • Solomon Adeleye Bakarey University of Ibadan, Nigeria

DOI:

https://doi.org/10.3126/jmcjms.v9i1.34419

Keywords:

Antibiotic resistance, Enterobacteriaceae, Extended Spectrum Beta-Lactamase

Abstract

Background and Objectives: Abuse and indiscriminate use of antibiotics, prolong hospital admission, travel history, organ transplants, immunocompromised conditions and age are parts of the factors that contribute to development of antibiotic resistance and intestinal carriage of Extended Spectrum Beta-Lactamases (ESBL) Enterobacteriaceae. These bacteria affect the course and outcome of an infection and continue to pose a challenge to infection management worldwide. This study was carried out to determine the antimicrobial susceptibility patterns and prevalence of ESBL-producing Enterobacteriaceae isolated from stool samples of HIV and AIDS patients in Ibadan, Nigeria.

Materials and Methods: One hundred stool samples were collected from consenting HIV and AIDS patients accessing care in Antiretroviral (ARV) Clinic in a secondary and a tertiary health care facility in Ibadan, Nigeria. Gram-negative bacteria were isolated and identified using conventional methods. Antimicrobial susceptibility test was carried out using the Kirby Bauer disc diffusion technique. Phenotypic detection of ESBL-producing isolates was carried out using Double Disc Synergy Test (DDST).

Results: A total of 240 Gram-negative bacteria were isolated comprising 100 (41.6%) Escherichia coli, 33(13.8%) Klebsiella pneumonia, 27(11.3%) Serratia marcescens, 20(8.3%) Salmonella enterica, 9(3.8%) Proteus vulgaris, 13(5.4%) Proteus mirabilis, 21(8.8%) Citrobacter freundii and 17(7.1%) Enterobacter aerogenes. Out these, 56 (23.3%) were ESBL-producers; comprising 23(41.0%) Escherichia coli, 9(16.0%) Klebsiella pneumonia, 6(10.7%) Serratia marcescens, 5(8.9%) Salmonella enterica, 3(5.3%) Proteus vulgaris, 5(8.9%) Proteus mirabilis, 2(3.6%) Citrobacter freundii and 3(5.4%) Enterobacter aerogenes. Among the ESBL producers, 45 (80.3%) and 38 (67.8%) showed resistance to trimethoprim/sulfamethoxazole and aztreonam while 3 (3.5%) showed resistance to ertapenem. Also, 96.4% (54/56) of the ESBL producers were multidrug resistant.

Conclusion: This study showed that HIV and AIDS patients are reservoirs of ESBL-producing Enterobacteriaceae through faecal carriage, presenting them as likely source of dissemination of ESBL producer in community and hospital settings.

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Author Biographies

Olutayo Israel Falodun, University of Ibadan, Nigeria

Department of Microbiology, Faculty of Science

Olayemi Ajayi, University of Ibadan, Nigeria

Department of Microbiology, Faculty of Science

Adewuyi Ebenezer Ademola, Caleb University, Imota, Lagos, Nigeria

2

Department of Biological Science and Biotechnology

Solomon Adeleye Bakarey, University of Ibadan, Nigeria

Institute for Advanced Medical Research and Training, College of Medicine

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Published

2021-07-08

How to Cite

Falodun, O. I., Ajayi, O., Ademola, A. E., & Bakarey, S. A. (2021). Antibiotic Susceptibility Patterns and Extended Spectrum Beta-Lactamase (ESBL) production in Enterobactericeae Isolated from Stool Samples of HIV and AIDS Patients in Ibadan, Nigeria. Janaki Medical College Journal of Medical Science, 9(1), 5–15. https://doi.org/10.3126/jmcjms.v9i1.34419

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Section

Research Articles