@article{Khanal_Rana_Raut_Dhakal_2019, title={Phytochemical Screening, Biological Studiesand GC-MS Analysis of Extract of Biden pilosa L}, volume={5}, url={https://www.nepjol.info/index.php/JMMIHS/article/view/24076}, DOI={10.3126/jmmihs.v5i1.24076}, abstractNote={<p><strong>Objective:</strong> The aim of the research work was to carry out the extraction of areal parts of <em>Biden pilosa L</em> by hydroalcholic and Hexane, ethyl acetate and acetone mixture followed by qualitative phytochemical analysis, acute oral toxicity test, anti-inflammatory test and GC-MS analysis of the extracts.</p> <p><strong>Method: </strong>The hydro-alcoholic and HEA(n-hexane, ethyl acetate and acetone) extraction was done from aerial parts using ethanol and water in the ratio 70:30 and n-hexane, ethyl acetate and acetone in the ratio of 1:1:1 (HEA extract)  respectively. Acute oral toxicity testwas performed OECD guidelines. The single spot in TLC was obtained using n-hexane as solvent for HEA fraction and finally phytocomponents were identified by GC-MS present in that spot.In vitro anti-inflammatory activity was performed by human RBC membrane stabilization method.</p> <p><strong>Result: </strong>The phytochemical test results obtained indicate that hydro-alcoholic extract of aerial part of <em>Bidens pilosa</em> L. possess alkaloids, tannins, terpenoids and saponins whereas HEA extract possess alkaloids, flavonoids, tannins. Both hydro-alcoholic and HEA extracts were found to be safe up to the dose of 2000 mg/kg BW of the mice. Both extracts showed significant in vitro anti-inflammatory activity in a concentration dependent manner. The GC-MS analysis of HEA extract of aerial parts showed the presence of the sixteen different compounds from partially separated extract from TLC plates.</p> <p><strong>Conclusion: </strong>Hydro-alcoholic extract of aerial part of <em>Bidens pilosa L</em>. possess alkaloids, tannins, terpenoids and saponins whereas HEA extract possess alkaloids, flavonoids, tannins. Both hydro-alcoholic and HEA extracts were found to be safe up to the dose of 2000 mg/kg BW of the mice. The GC-MS analysis of HEA extract of aerial parts showed the presence of the sixteen different compounds.</p>}, number={1}, journal={Journal of Manmohan Memorial Institute of Health Sciences}, author={Khanal, Dharma Prasad and Rana, Rupa and Raut, Bechan and Dhakal, Rabindra Prasad}, year={2019}, month={May}, pages={79–93} }