Molecular Confirmation of Salmonella typhimuriumin Poultry from Kathmandu Valley

  • Sanjeev Kumar Adhikari SANN International College, Purbanchal University, Gairidhara, Kathmandu
  • Arrogya Gyawali SANN International College, Purbanchal University, Gairidhara, Kathmandu
  • Sajan Shrestha SANN International College, Purbanchal University, Gairidhara, Kathmandu
  • Swoyam Prakash Shrestha Animal Health Research Division, Nepal Agricultural Research Council, Lalitpur, Nepal
  • Meera Prajapati Animal Health Research Division, Nepal Agricultural Research Council, Lalitpur, Nepal
  • Doj Raj Khanal Animal Health Research Division, Nepal Agricultural Research Council, Lalitpur, Nepal
Keywords: Salmonella typhimurium, invA, Invasion, Polymerase Chain Reaction (PCR)

Abstract

A prevalence study was carried to isolate Salmonella typhimurium from blood (n= 50) and gut samples (n=100) of poultry in Kathmandu valley during early 2016. Salmonella typhimurium bacteria isolated in the selective media were biochemically confirmed based on Bergey’s Manual. Two sets of oligonucleotide primers-the genus specific 16S rRNA and the organism specific invA were employed for molecular level confirmation by the Polymerase Chain Reaction (PCR) assay. The amplified fragments in 1% agarose gel observed at 406bp and 285bp, respectively confirmed the isolates to be Salmonella typhimurium. Of 150 samples tested, Salmonella typhimurium were isolated from 49 samples, among which nine were from blood (18%) and forty from the gut (40%). The present result indicated an alarmingly high level of Salmonella typhimurium, which can result inzoonotic infection in humans owing to increased contact with poultry and consumption of poultry products in the Kathmandu valley.

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Abstract
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Published
2018-05-06
How to Cite
Adhikari, S., Gyawali, A., Shrestha, S., Shrestha, S., Prajapati, M., & Khanal, D. (2018). Molecular Confirmation of Salmonella typhimuriumin Poultry from Kathmandu Valley. Journal of Nepal Agricultural Research Council, 4(1), 86-89. https://doi.org/10.3126/jnarc.v4i1.19694
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Articles