Molecular Confirmation of Salmonella typhimuriumin Poultry from Kathmandu Valley
AbstractA prevalence study was carried to isolate Salmonella typhimurium from blood (n= 50) and gut samples (n=100) of poultry in Kathmandu valley during early 2016. Salmonella typhimurium bacteria isolated in the selective media were biochemically confirmed based on Bergey’s Manual. Two sets of oligonucleotide primers-the genus specific 16S rRNA and the organism specific invA were employed for molecular level confirmation by the Polymerase Chain Reaction (PCR) assay. The amplified fragments in 1% agarose gel observed at 406bp and 285bp, respectively confirmed the isolates to be Salmonella typhimurium. Of 150 samples tested, Salmonella typhimurium were isolated from 49 samples, among which nine were from blood (18%) and forty from the gut (40%). The present result indicated an alarmingly high level of Salmonella typhimurium, which can result inzoonotic infection in humans owing to increased contact with poultry and consumption of poultry products in the Kathmandu valley.
Copyright (c) 2018 Nepal Agricultural Research Council
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright of articles is transferred to the Nepal Agricultural Research Council (NARC).
This journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. Users are allowed to
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
The Author warrants that the Work:
- Has not been published before (except if included in an institutional pre-print server).
- Is not presently being considered for publication elsewhere.
- Does not violate any intellectual property right of any person or entity.
- Does not contain any subject matter that contravenes any laws (including defamatory material and misleading and deceptive material) and
- Meets ethical standards applicable to the research discipline.
They should warrant that the work and all figures, illustrations, photographs, charts, and other supplementary material are original. They declare that this manuscript has not been published elsewhere and submitted, nor will be submitted, to any other publication while consideration by the JNARC. There is no any plagiarism in this manuscript and no part of this manuscript (referenced or otherwise) has been copied verbatim. No one who has contributed significantly to the work has been denied authorship and those who helped have been duly acknowledged. All authors reviewed and approved the content and parts of this manuscript as submitted.
Grant of pubishing rights
The authors should grant the publisher a license to publish their article, in all forms and all media (whether known at this time or developed at any time in the future) throughout the world, in all languages, where their rights include but are not limited to the right to translate, create adaptations, extracts, or derivative works and to sublicense such rights, for the full term of copyright (including all renewals and extensions of that term), to take effect if and when the article is accepted for publication. They confirm that they have read and accept the full Terms and Conditions below including author warranties, and have read and agree to comply with the Journal’s policies on peer review and publishing ethics.
Click to download Copyright Form.
Please read this agreement carefully, complete it, and return a copy to us by email, fax, or hard copy immediately, to avoid any delay in the publication of your article.