In-vitro Evaluation of Bio-control agents against Soil Borne Plant Pathogens

  • Shrinkhala Manandhar Nepal Agricultural Research Council https://orcid.org/0000-0002-9997-8900
  • Bimala Pant Nepal Agricultural Research Council
  • Chetana Manandhar Nepal Agricultural Research Council
  • Suraj Baidya Nepal Agricultural Research Council
Keywords: Biocontrol, Rhizosphere, Soil borne pathogens, Trichoderma

Abstract

Biocontrol is an important aspect of disease management for plant pathogens, especially for the soil borne fungi. Trichoderma species is the most exploited biocontrol agent in recent years. The soil specific nature of Trichoderma species is a well-known fact and hence native Trichoderma isolates should be more emphasized for control of plant pathogens. Fifty soil samples from rhizosphere of various agricultural crops were collected for isolation of Trichoderma sp. Ten isolates of Trichoderma were tested in dual culture with soil borne pathogens Fusarium solani, Rhizoctonia solani and Sclerotinia sclerotiorum in an in vitro assay. All of the test isolates were found to be significant in terms of mycelial inhibition growth as compared to control. However, varying degrees of antagonism by different Trichoderma isolates were observed for above mentioned soil borne pathogens. The isolate (T363) was found to exhibit more than 80% inhibition of S. sclerotiorum while the isolate T357 was found to control F. solani by more than 80%.  For the control of R. solani, six of the tested Trichoderma isolates showed more than 80% inhibition of its radial colony growth. The Trichoderma isolates seen effective in this study need to be tested in pot and field experiments for exploiting the use and benefits of biocontrol.

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Author Biographies

Shrinkhala Manandhar, Nepal Agricultural Research Council

Scientist, Plant Pathology Division

Bimala Pant, Nepal Agricultural Research Council

Scientist, Plant Pathology Division

Chetana Manandhar, Nepal Agricultural Research Council

Scientist, Plant Pathology Division

Suraj Baidya, Nepal Agricultural Research Council

Senior Scientist, Plant Pathology Division

Published
2019-04-28
How to Cite
Manandhar, S., Pant, B., Manandhar, C., & Baidya, S. (2019). In-vitro Evaluation of Bio-control agents against Soil Borne Plant Pathogens. Journal of Nepal Agricultural Research Council, 5(1), 68-72. https://doi.org/10.3126/jnarc.v5i1.23810
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Articles