@article{Subedi_Neupane_BK_Oli_2019, title={In-vitro Evaluation of Botanicals, Fungi-toxic Chemicals and Bio-control Agent for Efficacy against Turcicum Leaf Blight of Maize}, volume={5}, url={https://www.nepjol.info/index.php/JNARC/article/view/23811}, DOI={10.3126/jnarc.v5i1.23811}, abstractNote={<p>Maize is the second most important cereal crop of Nepal. Its growth and production is severely affected by Turcicum leaf blight caused by <em>Exserohilum turcicum</em> at pre-harvest stage. A total of 6 botanicals, 4 chemical fungicides and 1 bio-control agent were evaluated for efficacy against <em>Exserohilum turcicum </em>under <em>in vitro </em>conditions following poisoned food technique at National Maize Research Program, Rampur, Nepal. The experiment was carried out in a completely randomized design with 5 replications. All the tested botanicals, fungicides and bio-control agent exhibited fungicidal action and significantly inhibited mycelial growth of the test pathogen over untreated control. Among botanicals, extract of <em>Acorus calamus</em> L. at 1% W/V checked the pathogen growth completely <em>in-vitro</em>. The mycelial growth inhibition percent of <em>Artimisia indica </em>Willd, <em>Lantana camera</em> L., <em>Allium sativum</em> L<em>., Xanthoxylum armatum </em>DC., and <em>Azadirachta indica</em> A. Juss. at the concentration of 2.5% W/V on PDA was 75.18%, 74.00%, 44.68%, 44.21% and 37.59% respectively. Among fungicides, the mycelial growth inhibition percent of <em>E. turcicum</em> due to ACME-COP (Copper oxychloride 50% WP), SAAF (Carbendazim 12% + Mancozeb 63% WP), Dithane M-45 (Mancozeb 75% WP) and Bavistin (Carbendazim 50% WP) at the concentration of 1000 ppm on PDA was 70.69%, 68.44%, 61.23%, and 60.52% respectively. Antagonist <em>Trichoderma viride</em> developed more rapidly than <em>E. turcium </em>in single as well as in dual cultures. <em>T. viride </em>caused significantly inhibition of 35% of the pathogen <em>E. turcicum </em>on the 5<sup>th</sup> day of incubation. These results have important implications for the management of turcicum leaf blight disease in maize.</p>}, number={1}, journal={Journal of Nepal Agricultural Research Council}, author={Subedi, Subash and Neupane, Saraswati and BK, Surendra and Oli, Lokendra}, year={2019}, month={Apr.}, pages={73–80} }