@article{Gyawali_Ghimire_Khatiwada_Niraula_Sharma_Thapa_2020, title={Anti-inflammatory, Anxiolytic and Antioxidant Property of Lactuca sativa L and Formulation of Microspheres Loaded Sustained Release Anti-inflammatory Gel}, volume={41}, url={https://www.nepjol.info/index.php/JNCS/article/view/30371}, DOI={10.3126/jncs.v41i1.30371}, abstractNote={<p>Anti-inflammatory and anxiolytic properties of <em>Lactuca sativa</em> L extract was evaluated by formalin induced mice paw edema test, and elevated plus maze animal model respectively. Solid microspheres containing dispersed extract were obtained by water-in-oil-in-water multiple emulsion system followed by solvent evaporation method. Optimization was carried out using MINITAB 16 and optimized microspheres were loaded into a gel base. Anti-oxidant activity of optimized microspheres loaded gel was evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. The gel was also evaluated for its physical properties, drug release kinetics and anti-inflammatory activity using formalin induced mice-paw edema test where the anti-inflammatory potential was compared to marketed diclofenac gel (1.16% w/w). All doses of extract as well as microsphere loaded sustained release anti-inflammatory gel showed a dose and time dependent inhibition of edema (P<0.05), the extract also showed a dose dependent inhibition of level of anxiety (P<0.001). Potential anti-oxidant activity was seen with different concentration of crude extract and microsphere loaded gel. The anti-oxidant potential was compared using IC<sub>50</sub> value which was 5.62 µg/mL, 6.34 µg/ml, 8.25 µg/mL for ascorbic acid, methanolic extract of <em>L. sativa</em> and microspheres loaded gel respectively.</p>}, number={1}, journal={Journal of Nepal Chemical Society}, author={Gyawali, Rajendra and Ghimire, Anupama and Khatiwada, Apeksha and Niraula, Prakshit and Sharma, Ushma and Thapa, Roshani}, year={2020}, month={Jul.}, pages={8–15} }