@article{Karn_Bhattarai_Rauniyar_Adhikari_Karna_Upadhyay_Gupta_2018, title={Determination of CD4+ T- Lymphocytes in Healthy Children of Kathmandu}, volume={16}, url={https://www.nepjol.info/index.php/JNHRC/article/view/21432}, abstractNote={<p><strong>Background:</strong> The cluster differentiation (CD) of T-cell is the good marker for the immunological competence study. Nepal does not have a reference value for CD4+ T cell count and percentage for children, which severely limits the prospect of pediatric prognosis.</p><p><strong>Methods:</strong> This cross-sectional study was conducted in Kathmandu valley where total 207 children of age 0-14 year age group were recruited in this study. We analyzed 50 cord blood and 157 peripheral blood samples in order to calculate the absolute count of CD4+ T lymphocyte using Fluorescence-activated cell sorting methodology.</p><p><strong>Results:</strong> The reference range for absolute CD4+ T cell count was found to be 634-4040 cells/µL (mean1470; median: 1335 and 95% CI [1322-1617]) for male children and 491-2922 cells/µL (mean: 1443 median: 1326 and 95% CI [1298-1588]) for the female children. We also observed elevated CD4 to the CD3 ratio in younger children (0.67 from cord blood Vs 0.53 from 10-14yr) compared to older ones.</p><p><strong>Conclusions:</strong> The observed CD4+ T cell counts among healthy children of Kathmandu highlights the gender differences skewed for male as well the need of defining specific reference values for other lymphocyte subsets as well in a country like Nepal which has a population with diverse genetic and socio-cultural parameters.</p>}, number={3}, journal={Journal of Nepal Health Research Council}, author={Karn, Sapana and Bhattarai, Manjula and Rauniyar, Ramanuj and Adhikari, Anurag and Karna, Pratik and Upadhyay, Bishnu Prasad and Gupta, Birendra Prasad}, year={2018}, month={Nov.}, pages={325–329} }