Study of Extended Spectrum Beta-Lactamases Producing Escherichia Coli and Klebsiella Species in a Tertiary Care Hospital, Biratnagar, Nepal

  • Kumari Ragani Yadav Department of Microbiology, Nobel Medical College Teaching Hospital Biratnagar https://orcid.org/0000-0003-0038-473X
  • Ganesh kumar Singh Department of Microbiology, Nobel Medical College Teaching Hospital Biratnagar
  • Sujit Bhattacharjee Department of Microbiology, Nobel Medical College Teaching Hospital Biratnagar
  • Kewal Shrestha Department of Microbiology, Nobel Medical College Teaching Hospital Biratnagar
Keywords: Antibiotic, Escherichia coli, Urinary Tract Infections

Abstract

Background: Escherichia coli and Klebsiella species are most common ESBL producers and associated with UTI in both community and hospital setting, often limiting the treatment therapy of UTI. Aim of present study was Isolation and Identification of ESBL producing E. coli and Klebsiella Species in urine sample from cases of UTI and their antibiotic susceptibility pattern at Nobel Medical College Teaching Hospital, Biratnagar, Nepal.

Materials and Methods: This descriptive cross-sectional study was conducted at Nobel Medical College with effect from October 2018 to June 2019 after approval from the Institutional Review Committee (IRC) of the college as per clinical laboratory standards institute of (CLSI) guidelines.

Results: Out of total 2567 urine samples, 631(24.5%) samples showed microbial growth. Among which E. coli was 288(45.6%), Klebsiella species were 20(31.5%), other gram-negative bacteria were 158(25%), gram positive bacteria were 114(18%) and fungus 51(8%) were recovered. Out of 631 culture positive urine samples, 308 urine samples were positive for E. coli/K. species 288(93.5%)/20(42.5%) respectively. Among which ESBL isolates were 213(69%) and non ESBL isolates were 95(31%). All ESBL producing E. coli and K. species were found (100%) sensitive to meropenem. Similarly, among other antibiotics also showed increased susceptibility towards the piperacillin/tazobactum (97.2%),cefoperazone/sulbactum (94.4%) and amikacin (93.0%).

Conclusion: The highest numbers of ESBL producers E. coli and K. species from urine sample are increasing day by day and creating serious problem in treating patients in Nepal. So it should be mandatory and very essential to have routine monitoring system to detect the ESBL producing isolates in clinical laboratories.

Downloads

Download data is not yet available.
Abstract
14
PDF
20

Author Biography

Kumari Ragani Yadav, Department of Microbiology, Nobel Medical College Teaching Hospital Biratnagar

Lecturer

Published
2019-12-16
How to Cite
Yadav, K., Singh, G., Bhattacharjee, S., & Shrestha, K. (2019). Study of Extended Spectrum Beta-Lactamases Producing Escherichia Coli and Klebsiella Species in a Tertiary Care Hospital, Biratnagar, Nepal. Journal of Nobel Medical College, 8(2), 31-36. https://doi.org/10.3126/jonmc.v8i2.26743
Section
Original Articles