TY - JOUR AU - Chaudhary, Pradip Kumar AU - Shrestha, Mitesh AU - Poudel, Bal Hari AU - Adhikari, Mahesh Kumar PY - 2017/12/31 Y2 - 2024/03/28 TI - In vitro Cultivation of Newly Reported Wild Edible Mushroom Volvariella bomybycina from Nepal JF - Nepal Journal of Biotechnology JA - Nepal J Biotechnol VL - 5 IS - 1 SE - Original Research Articles DO - 10.3126/njb.v5i1.18867 UR - https://www.nepjol.info/index.php/NJB/article/view/18867 SP - 27-31 AB - <p>Wild edible mushrooms are becoming endangered all over the world. Very few wild edible mushrooms are found in natural habitat. <em>Volvariella bombycina </em>is an edible and medicinal mushroom. The mushroom was collected in natural habitat growing on <em>Populus </em>tree. Mycelium of the mushroom was developed in PDA slant tubes by tissue culture method, incubated at 25°C for 1-2 weeks. Spawn was developed in wheat grains after incubation at 25°C for 2-3 weeks. Substrates were formulated for the development of fruiting bodies by combination of paddy straw, saw dust and rice husk. Fruiting bodies of <em>V. bombycina </em>was cultivated in these substrates after incubation at 28 ± 2°C for 2-4 weeks. The work describes the optimized process for in vitro culture of wild edible mushroom <em>Volvariella bomybycina</em>.</p><p>Nepal Journal of Biotechnology. Dec. 2017 Vol. 5, No. 1: 27-31</p> ER -