Estimation of Total Phenolic and Flavonoid Contents in Some Medicinal Plants and Their Antioxidant Activities

Authors

  • Bedraj Pandey Central Department of Chemistry, Tribhuvan University, Kathmandu
  • Meena Rajbhandari Research Center for Applied Science and Technology, Tribhuvan University, Kathmandu

DOI:

https://doi.org/10.3126/njst.v15i1.12010

Keywords:

Antioxidant activity, Gallic acid, Medicinal plants, Quercetin, Total flavonoids phenolics

Abstract

Quercetin was isolated from ethyl acetate fraction of a methanol extract of the outer scale of onion by repeated sephadex LH-20 column chromatography. Methanol, 50% aqueous methanol and 70% aqueous acetone extracts of different parts of four medicinal plants, Ficus benghalensis, Elaeocarpus sphaericus, Ipomea carnea and Azeratum conyzoides were prepared and screened for total phenolic and flavonoid contents and free radical scavenging activity. The total phenolics contents were measured spectrophotometrically by using Folin-Ciocalteu reagent and total flavonoids content by using aluminum chloride colorimetric method. Free radical scavenging activity was evaluated using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. All the investigated medicinal plant extracts contained high amount of phenolics. The highest amount was detected in 70% aqueous acetone extract of E. sphaericus (298.8±9.03 mg GAE/g extract) and lowest amount in 50% aqueous methanol extract of F. benghalensis (6.7±0.73 mg GAE/g extract). The highest amount of flavonoid was found in methanol extract (78.2±2.72 mg quercetin/g extract) and the lowest amount was detected in 50% aqueous methanol extract (2.1±0.25 mg quercetin/g extract) of F. benghalensis. DPPH free radical scavenging assay was carried out only in 70% acetone extracts. The highest IC50 value was observed for E. sphaericus (34.0 μg/ml). A strong linear correlation between total phenolic content and antioxidant activity was found indicating that the major antioxidants are phenolics.

DOI: http://dx.doi.org/10.3126/njst.v15i1.12010

 

Nepal Journal of Science and Technology

Vol. 15, No.1 (2014) 53-60

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Published

2015-02-01

How to Cite

Pandey, B., & Rajbhandari, M. (2015). Estimation of Total Phenolic and Flavonoid Contents in Some Medicinal Plants and Their Antioxidant Activities. Nepal Journal of Science and Technology, 15(1), 53–60. https://doi.org/10.3126/njst.v15i1.12010

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Articles