Biological Activities of Three Different Medicinal Plants from Himalayan Region of Nepal

Authors

  • Keshab Bhattarai Department of Biotechnology, Kathmandu University, Dhulikhel
  • Tirtha Maiya Shrestha Department of Pharmacy, Kathmandu University, Dhulikhel
  • Ramita Bajracharya Department of Pharmacy, Kathmandu University, Dhulikhel
  • SC Jain Department of Chemistry, University of Delhi, Delhi
  • Janardan Lamichhane Department of Biotechnology, Kathmandu University, Dhulikhel

DOI:

https://doi.org/10.3126/njst.v11i0.4136

Keywords:

antibacterial, antiproliferative, microtitration, HeLa cells

Abstract

Crude chloroform/methanol and methanol extracts of leaves and twigs of three medicinal plants collected from different altitudes of Nepal, Rhododendron lepidotum, Hippophae rhamnoids and Cornus capitata were examined for their antibacterial, cytotoxicity and antiproliferative activities. The antimicrobial activity shown by methanol extracts of R. lepidotum and C. capitata against Staphylococcus aureus was comparable with that of streptomycin antibiotic whereas the activity of the extract of H. rhamnoids was very low. Cytoxicity (LC50) values ranged from 46.4 to 111.6 μg/ml with R. lepidotum and 129.4 to 464.1 μg/ml with C. capitata indicating high cytoxic property in the extract of R. lepidotum and medium in C. capitata. Similarly, anti-proliferative activity of R. lepidotum against HeLa cell line was relatively high activity in comparison to C. capitata and H. rhamnoids. Phytochemical screening revealed the presence of flavonoids, tannins, terpenoids, steroids, glycosides and coumarins, which could be responsible for the bioactivities shown by these plants.

Key words: antibacterial; antiproliferative; microtitration; HeLa cells

DOI: 10.3126/njst.v11i0.4136

Nepal Journal of Science and Technology 11 (2010) 139-146

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How to Cite

Bhattarai, K., Shrestha, T. M., Bajracharya, R., Jain, S., & Lamichhane, J. (2011). Biological Activities of Three Different Medicinal Plants from Himalayan Region of Nepal. Nepal Journal of Science and Technology, 11, 139–146. https://doi.org/10.3126/njst.v11i0.4136

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