Estimation of Alkaloids and Antibacterial Activity of Aconitum spicatum Bruhl Stapf from Manaslu Conservation Area

Authors

  • Sajan L Shyaula Nepal Academy of Science and Technology, Khumaltar, Lalitpur
  • Ash B Ngakushi Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu
  • Bijaya L Maharjan Nepal Academy of Science and Technology, Khumaltar, Lalitpur
  • Mangala D Manandhar Nepal Academy of Science and Technology, Khumaltar, Lalitpur

DOI:

https://doi.org/10.3126/njst.v13i1.7443

Keywords:

Aconitum spicatum, antibacterial, Manaslu conservation area, total alkaloid

Abstract

Aconitum spicatum is one of the deadly poisonous and highly valued medicinal plant. It is a national prioritized herb among 30 national priority herbs listed by the government of Nepal for their development, research and cultivation. The acute toxicity of the extract of this plant appeared to be directly related to the alkaloid content. The amount of total alkaloid of A. spicatum collected from Manaslu conservation area was calculated to be 1.7% by spectrophotometric method based on Dragendorff’s reagent. In-vitro evaluation of the crude extract of plant rhizomes using agar well diffusion assay against eight bacteria, Staphylococcus aureus, Escherichia coli, Schigella flexneri, Pseudomonas aeruginosa, Salmonella typhi, Salmonella paratyphi, Salmonella typhimurium and Klebsiella pneumonia, displayed potential antibacterial activity. The diethyl ether fraction was the most effective against all pathogenic microbes with minimum bacterial concentration value 3.125-6.25 mg/ml.

Nepal Journal of Science and Technology Vol. 13, No. 1 (2012) 67-71

DOI: http://dx.doi.org/10.3126/njst.v13i1.7443

Downloads

Download data is not yet available.
Abstract
870
PDF
1298

Downloads

Published

2013-01-21

How to Cite

Shyaula, S. L., Ngakushi, A. B., Maharjan, B. L., & Manandhar, M. D. (2013). Estimation of Alkaloids and Antibacterial Activity of Aconitum spicatum Bruhl Stapf from Manaslu Conservation Area. Nepal Journal of Science and Technology, 13(1), 67–71. https://doi.org/10.3126/njst.v13i1.7443

Issue

Section

Articles