@article{Maharjan_Thakuri_Thapa_Pradhan_Pant_Joshi_Pant_2020, title={In vitro propagation of the endangered orchid Dendrobium chryseum Rolfefrom protocorms culture}, volume={19}, url={https://www.nepjol.info/index.php/NJST/article/view/29737}, DOI={10.3126/njst.v19i1.29737}, abstractNote={<p>The immature seeds of <em>Dendrobium chryseum, </em>a sympodial epiphytic orchid with yellow flowers, were cultured <em>in vitro, </em>and the resultant protocorms were used as explants for seedling development. Protocorms were cultured on ½ MS medium fortified with Kinetin (Kn), 6-Benzylaminopurine (BAP), and Gibberellic Acid (GA3) in three concentrations (0.5mg/L, 1.0 mg/L and 2.0 mg/L) both alone and supplemented with 5% and 10% coconut water (CW). The highest number of shoots of D. chryseum developed on ½ - MS medium fortified with 2.0 mg/L of Kn and 10% CW and the longest shoots developed on ½ MS media fortified with 1.0 mg/L GA3, and 10% CW. The shoot derived from protocorms were placed in ½ MS medium fortified with three different rooting hormones, Indole -3- acetic acid (IAA), Indole -3-butyric acid (IBA) and α-Naphthalene acetic acid (NAA) in different concentrations alone as well as with each 1.0 mg/L hormone combined with 10% CW. The most effective of these media was ½ MS medium fortified with 1.5 mg/L IAA for rooting as well as for the production of longest roots. The present study could be useful for standardizing the protocol for mass propagation of the endangered orchid <em>Dendrobium chryseum. </em></p> <p> </p>}, number={1}, journal={Nepal Journal of Science and Technology}, author={Maharjan, Sabitri and Thakuri, Laxmi Sen and Thapa, Bir Bahadur and Pradhan, Shreeti and Pant, Krishna K. and Joshi, Giri P. and Pant, Bijaya}, year={2020}, month={Jun.}, pages={39–47} }