TY - JOUR AU - Thapa, Chandra Bahadur AU - Pant, Krishna Kumar AU - Bhattarai, Hari Datta AU - Pant, Bijaya PY - 2022/12/31 Y2 - 2024/03/28 TI - In vitro Induction and Proliferation of Callus in Piper longum L. through Leaf Culture JF - Nepal Journal of Science and Technology JA - Nepal Journal of Science and Technology VL - 21 IS - 1 SE - Articles DO - 10.3126/njst.v21i1.49892 UR - https://www.nepjol.info/index.php/NJST/article/view/49892 SP - 13-22 AB - <p><em>Piper longum</em> L. (Family Piperaceae) is a well- known health promoter used to treat cough, chronic bronchitis, asthma, and diabetes mellitus. The study is aimed to develop a protocol for callus induction and proliferation in <em>P. longum</em>. The leaf explants from mature plants were cultured on an MS basal medium supplemented with various concentrations of plant growth hormones, viz. 2,4-Dichlorophenoxyacetic acid (2, 4-D), Kinetin (KN), 6-Benzylaminopurine (BAP), and α-Naphthalene acetic acid (NAA), as well as 10% coconut water. In primary culture, the callus was compact and light white. The best callus induction and growth were observed in the MS basal medium containing1.0 mg/L 2,4-D+ 2.0 mg/L KN at 12 weeks of primary culture. At eight weeks of secondary culture, the MS medium containing 2.0 mg/L BAP alone and 0.5 mg/L NAA + 2.0 mg/L BAP and 10% coconut water had the best callus proliferation. Compared to 2,4-D and KN alone, BAP alone supported rapid callus growth in the MS medium. In <em>P. longum</em>, large-scale callus formation from leaf explants could be exploited to produce, isolate, and increase bioactive secondary metabolites for therapeutic purposes.</p> ER -