TY - JOUR AU - Bajracharya, Gan B AU - Maharjan, Rokesh PY - 2013/10/14 Y2 - 2024/03/29 TI - Cytotoxicity, Total Phenolic Content and Antioxidant Activity of Bergenia purpurascens Rhizome JF - Nepal Journal of Science and Technology JA - Nepal Journal of Science and Technology VL - 14 IS - 1 SE - Articles DO - 10.3126/njst.v14i1.8927 UR - https://www.nepjol.info/index.php/NJST/article/view/8927 SP - 87-94 AB - <p>This study was aimed to evaluate the cytotoxicity of methanolic extract (ME) and aqueous extract (AE) of the rhizomes of <em>Bergenia purpurascens </em>through the Brine-shrimp bioassay, which revealed that ME possessed cytotoxicity with LC<sub>50</sub> = 181.97 &igrave;g/ml value. The plant extracts were also evaluated <em>in vitro </em>for antioxidant activity using different redox [Folin-Ciocalteu (FC) and Ferric Reducing Antioxidant Power (FRAP)] and radical scavenging [(2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&rsquo;-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)] assays. Both ME and AE possessed antioxidant activity and ME displayed comparatively a greater potential. The total phenolic contents in ME and AE was 1.27 and 0.06 g gallic acid equivalent (GAE)/100 g dry mass (DM), respectively. The FRAP values determined for ME and AE were 15.82 and 2.58 &igrave;mol Fe(II)/100 g DM, respectively. The DPPH radical scavenging effect (IC<sub>50</sub>) was for ME 19.86 &igrave;g/ml and 295.91 &igrave;g/ml for AE. At 100 &igrave;g/ml concentration, up to 94% inhibition of ABTS<sup>+</sup> was observed in 6 min incubation.</p> <p>Nepal Journal of Science and Technology Vol. 14, No. 1 (2013) 87-94</p> <p>DOI: <a href="http://dx.doi.org/10.3126/njst.v14i1.8927">http://dx.doi.org/10.3126/njst.v14i1.8927</a></p> ER -