Study of Antimicrobial Activity of Lime Juice against Vibrio cholerae

Authors

  • Bina Laxmi Jayana Nepal Academy of Science and Technology, Khumaltar, Lalitpur
  • Tista Prasai Nepal Academy of Science and Technology, Khumaltar, Lalitpur
  • Anjana Singh Central Department of Microbiology, Tribhuvan University, Kathmandu
  • Kayo Devi Yami Nepal Academy of Science and Technology, Khumaltar, Lalitpur

Keywords:

Agar well, Crude extract, Minimum bactericidal concentration, Zone of inhibition

Abstract

 

The antimicrobial effect of crude extract of lime (Citrus aurantifolia) was assayed against different bacterial species by agar well diffusion method. The highest inhibition zone of 28 mm was observed in Vibrio cholerae followed by Enterobacter species (9mm), Citrobacter species (8mm) and Escherichia coli (8mm). Shigella, Salmonella and Klebsiella species were found resistant. So the study was mainly focused on V. cholerae. Different concentrations of the crude extract such as 75%, 50%, 25% and 5% were tested to calculate the minimum amount that inhibits the bacteria which showed the zone of inhibition (ZOI) as 31mm, 24mm, 17mm and 9mm respectively. The minimum bactericidal concentration (MBC) ranged from 6.25 mg/l to 50 mg/l. This result suggests that citrus fruit like limes are effective in preventing infection with Vibrio species.

Key words: Agar well; Crude extract; Minimum bactericidal concentration; Zone of inhibition.

DOI: 10.3126/sw.v8i8.3847

Scientific World Vol.8(8) 2010 pp.44-46

 

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How to Cite

Study of Antimicrobial Activity of Lime Juice against Vibrio cholerae. (2010). Scientific World, 8(8), 44-46. https://doi.org/10.3126/sw.v8i8.3847

Issue

Vol. 8 No. 8 (2010)

Section

Articles

How to Cite

Study of Antimicrobial Activity of Lime Juice against Vibrio cholerae. (2010). Scientific World, 8(8), 44-46. https://doi.org/10.3126/sw.v8i8.3847