Isolation and screening of thermophilic cellulolytic bacteria from compost piles

Authors

  • A Acharya Central Department of Microbiology, Tribhuvan university, Kirtipur
  • DR Joshi Central Department of Microbiology, Tribhuvan university, Kirtipur
  • K Shrestha Natural Product Chemistry Research Laboratory, Nepal Academy of Science and Technology (NAST) Khumaltar, Lalitpur,
  • DR Bhatta Central Department of Microbiology, Tribhuvan university, Kirtipur

DOI:

https://doi.org/10.3126/sw.v10i10.6861

Keywords:

Thermophilic cellulolytic bacteria, Cellulase enzyme, Compost piles

Abstract

Cellulose, a major polysaccharide found in agricultural residues and industrial and municipal wastes. In the present study thermophilic cellulolytic microorganisms were isolated. The isolates were tested for their cellulytic activity. The enzyme production from potent isolates was optimised using cellulose basal broth medium. Activity of partially purified enzyme was determined. The most potent thermophilic cellulolytic isolates were identified as Bacillus subtilis. The crude cellulase enzyme concentrated at 80-85% ammonium sulfate produced highest zone of hydrolysis. The enzymatic degradation of cellulose waste has been suggested as a feasible alternative for the conversion of lignocelluloses substrate into fermentable sugars and application for biofuel production.

Scientific World, Vol. 10, No. 10, July 2012 p43-46

DOI: http://dx.doi.org/10.3126/sw.v10i10.6861

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Published

2012-09-20

How to Cite

Acharya, A., Joshi, D., Shrestha, K., & Bhatta, D. (2012). Isolation and screening of thermophilic cellulolytic bacteria from compost piles. Scientific World, 10(10), 43–46. https://doi.org/10.3126/sw.v10i10.6861

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Articles