TY - JOUR AU - Kafle, Saroj AU - Basnyat, Ram Chandra AU - Rajbhandari, Meena PY - 2021/02/16 Y2 - 2024/03/28 TI - Phytochemical Analysis, Antioxidant, Antimicrobial Activities and GC-MS Profiling Of Drymaria Diandra Blume JF - Scientific World JA - Sci World VL - 14 IS - 14 SE - Articles DO - 10.3126/sw.v14i14.35000 UR - https://www.nepjol.info/index.php/SW/article/view/35000 SP - 90-98 AB - <p><em>Drymaria diandra</em> is used in Nepalese traditional medicine to treat various ailments. The hexane, dichloromethane, ethyl acetate, methanol and 50% aqueous methanol extracts of <em>D. diandra</em> were prepared and screened for the presence of different classes of phytochemicals. Methanol and 50% aqueous methanol extracts showed the presence of phenolics, flavonoids and carbohydrates so their total contents were estimated. The highest amount of phenolics and flavonoids were found in methanol extract (190.58±2.21 mg GAE/g extract and 69.25±7.91 mg CE/g extract respectively). The highest amount of sugar was found in 50% methanol extract (185.60±2.53 mg GE/g extract). Similarly, the extracts were tested for the antibacterial activity against one gram negative bacteria, Escherichia coli and one gram positive bacteria <em>Staphylococcus aureus</em>. All the tested extracts showed good antibacterial activity against both bacteria with the inhibition zone ranging from 10 -22 mm. The free radical scavenging activity was determined using DPPH free radical. Both methanol and 50% aqueous methanol extracts showed strong antioxidant activity (IC<sub>50</sub> 13.61 and 16.94 μg/ml respectively). The GC-MS analysis of hexane extract revealed the presence of more than 22 compounds. However, 9 compounds were identified by comparing the mass fragmentation pattern of each compound with the standard NIST mass spectral database</p> ER -