Biofilm Production by Uropathogens like Klebsiella spp and Pseudomonas spp and their Antibiotic Susceptibility

  • Sanjay Mahato Mahendra Morang Adarsha Multiple Campus https://orcid.org/0000-0002-0154-8129
  • Pinki Mandal Mahendra Morang Adarsha Multiple Campus
  • Ajay Mahato Birat Medical College and Teaching Hospital
Keywords: Antibiotic Susceptibility,Biofilm, Klebsiella, Pseudomonas,Uropathogen

Abstract

Introduction: Urinary Tract Infection (UTI) causes inflammation which is a common, painful and sometimes life-threatening condition as well. Despite high prevalence of bacteriuria, the information on biofilm forming bacteria is negligible.

Objectives: This study aims at understanding the status of the biofilm forming nature of Klebsiella spp and Pseudomonas SPP and their drug resistance property with several class of antibiotics with a prime focus on resistance pattern against few penicillin based drugs to few empirical drugs in today's time.

Methodology: Urine samples were analyzed and the isolates were biochemically identified. Then, the isolates were tested for several drugs so as to identify multidrug resistance nature of isolates by Kirby-Bauer Disc method. Biofilm forming nature was examined on Congo Red Agar.

Results Out of 35 urine samples, 13 isolates (37.1%) were found to be positive with significant bacteriuria. Eight samples (22.8%) showed incidence of Klebsiella spp. and 5 samples (14.3%) showed P. aeruginosa. The prevalence of Klebsiella spp. (Klebsiella pneumoniae (46.2%) and K. oxytoca(15.4%)) and P. aeruginosa was found to be 61.4% and 38.6% respectively. 66.6% of K. pneumoniae and 50% K. oxytocawere biofilm forming pathogen.K. pneumoniae andK. oxytoca were resistant to amoxycillin, amoxycillin-clavulanate, and cefoxitin; while were sensitive to nitrofurantoin and azithromycin.P. aeruginosa were sensitive to azithromycin(100%), but showed 60% resistance to levofloxacin and ofloxacin. Eight (61.5%) isolates were found to be MDR. 100% of Klebsiella oxytoca (n=2), 66.7% of Klebsiella pneumoniae (n=4), and 40% of Pseudomonas aeruginosa (n=2)were multidrug resistant (MDR).Multiple antibiotic resistance (MAR) indices of bacteria revealed that all the 13 isolates were Multi-Antibiotics Resistance strains.

Conclusion Biofilm forming nature is now much greater in Klebsiella spp; while most of the isolates like Klebsiella and Pseudomon as are multidrug resistant.

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Author Biographies

Sanjay Mahato, Mahendra Morang Adarsha Multiple Campus

Lecturer, Department of Microbiology,

Pinki Mandal, Mahendra Morang Adarsha Multiple Campus

Student, Department of Microbiology,

Ajay Mahato, Birat Medical College and Teaching Hospital

Lecturer, Department of Orthopaedics

Published
2020-06-26
How to Cite
Mahato, S., Mandal, P., & Mahato, A. (2020). Biofilm Production by Uropathogens like Klebsiella spp and Pseudomonas spp and their Antibiotic Susceptibility. Birat Journal of Health Sciences, 5(1), 902-906. https://doi.org/10.3126/bjhs.v5i1.29609
Section
Original Research Articles