@article{Mahato_Mandal_Mahato_2020, title={Biofilm Production by Uropathogens like Klebsiella spp and Pseudomonas spp and their Antibiotic Susceptibility}, volume={5}, url={https://www.nepjol.info/index.php/bjhs/article/view/29609}, DOI={10.3126/bjhs.v5i1.29609}, abstractNote={<p><strong>Introduction: </strong>Urinary Tract Infection (UTI) causes inflammation which is a common, painful and sometimes life-threatening condition as well. Despite high prevalence of bacteriuria, the information on biofilm forming bacteria is negligible.</p> <p><strong>Objectives: </strong>This study aims at understanding the status of the biofilm forming nature of <em>Klebsiella </em>spp and <em>Pseudomonas </em>SPP and their drug resistance property with several class of antibiotics with a prime focus on resistance pattern against few penicillin based drugs to few empirical drugs in today’s time.</p> <p><strong>Methodology</strong>: Urine samples were analyzed and the isolates were biochemically identified. Then, the isolates were tested for several drugs so as to identify multidrug resistance nature of isolates by Kirby-Bauer Disc method. Biofilm forming nature was examined on Congo Red Agar.</p> <p><strong>Results </strong>Out of 35 urine samples, 13 isolates (37.1%) were found to be positive with significant bacteriuria. Eight samples (22.8%) showed incidence of <em>Klebsiella </em>spp. and 5 samples (14.3%) showed <em>P. aeruginosa</em>. The prevalence of <em>Klebsiella </em>spp. (<em>Klebsiella pneumoniae </em>(46.2%) and <em>K. oxytoca</em>(15.4%)) and <em>P. aeruginosa </em>was found to be 61.4% and 38.6% respectively<em>. </em>66.6% of <em>K. pneumoniae </em>and 50% <em>K. oxytoca</em>were biofilm forming pathogen.<em>K. pneumoniae </em>and<em>K. oxytoca </em>were resistant to amoxycillin, amoxycillin-clavulanate, and cefoxitin; while were sensitive to nitrofurantoin and azithromycin.<em>P. aeruginosa </em>were sensitive to azithromycin(100%), but showed 60% resistance to levofloxacin and ofloxacin. Eight (61.5%) isolates were found to be MDR. 100% of <em>Klebsiella oxytoca </em>(n=2), 66.7% of <em>Klebsiella pneumoniae </em>(n=4), and 40% of <em>Pseudomonas aeruginosa </em>(n=2)were multidrug resistant (MDR).Multiple antibiotic resistance (MAR) indices of bacteria revealed that all the 13 isolates were Multi-Antibiotics Resistance strains.</p> <p><strong>Conclusion </strong>Biofilm forming nature is now much greater in <em>Klebsiella </em>spp; while most of the isolates like <em>Klebsiella </em>and <em>Pseudomon as </em>are multidrug resistant.</p>}, number={1}, journal={Birat Journal of Health Sciences}, author={Mahato, Sanjay and Mandal, Pinki and Mahato, Ajay}, year={2020}, month={Jun.}, pages={902–906} }