Phytochemical Profiling and Bioactivity Assessment of Colebrookea oppositifolia Sm. from the High Altitudes of Nepal's Far Western Region

Authors

  • Anil Ojha Department of Physics, Far Western University, Kailali Multiple Campus
  • Prakash Raj Pant Department of Physics, Far Western University, Kailali Multiple Campus
  • Sabina Kumari Dhami Department of Physics, Far Western University, Kailali Multiple Campus
  • Naresh Singh Dhami Department of Chemistry, Far Western University, Kailali Multiple Campus
  • Prem Raj Joshi Department of Physics, Tribhuvan University
  • Aarati Bhatta Department of Biology, Far Western University, Kailali Multiple Campus
  • Nirajan Bahadur Singh Department of Biology, Far Western University, Kailali Multiple Campus
  • Gunanand Pant Department of Biology, Far Western University, Kailali Multiple Campus
  • Tek Raj Ojha St. Xavier College, Tribhuvan University
  • Gajendra Pant Department of Chemistry, Far Western University, Kailali Multiple Campus
  • Prem Singh Saud Department of Chemistry, Far Western University, Kailali Multiple Campus

DOI:

https://doi.org/10.3126/fwr.v3i2.92803

Keywords:

antibacterial, TFC, TPC, Colebrookea oppositifolia Sm.

Abstract

Traditional methods have made considerable use of herbal therapy to treat a variety of illnesses. This study aimed to quantify the levels of phenolic compounds and flavonoids in extracts made with two distinct solvents and evaluate their antibacterial qualities. Methanolic extract exhibited the highest concentration of bioactive compounds, with a total phenolic content (TPC) of 125.68 ± 2.67 mg gallic acid equivalents (GAE) per gram and a total amount of flavonoids (TFC) of 35.28 ± 3.22 mg quercetin equivalents (QE) per gram. Antibacterial assays revealed that both methanolic and extracts of hexane extracts efficiently inhibited Shigella sonnei, producing a zone of inhibition measuring 12 mm. These findings underscore the significant bioactive potential of the plant extracts under investigation, particularly those obtained using methanol and hexane solvents. Such properties render them promising candidates for the development of novel therapeutic agents aimed at treating infectious diseases.

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Published

2025-12-31

How to Cite

Ojha, A., Pant, P. R., Dhami, S. K., Dhami, N. S., Joshi, P. R., Bhatta, A., … Saud, P. S. (2025). Phytochemical Profiling and Bioactivity Assessment of Colebrookea oppositifolia Sm. from the High Altitudes of Nepal’s Far Western Region. Far Western Review, 3(2), 1–10. https://doi.org/10.3126/fwr.v3i2.92803

Issue

Vol. 3 No. 2 (2025)

Section

Articles

License

Creative Commons License

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