@article{Shrestha_Thapa_Dahal_Adhikari_Shrestha_2018, title={Microbial Profile of Various Catheter Tips among Hospitalized Patients}, volume={5}, url={https://www.nepjol.info/index.php/tujm/article/view/22299}, DOI={10.3126/tujm.v5i0.22299}, abstractNote={<p><strong>Objectives: </strong>This study aimed to identify the microbiological profile of various catheter tips, and multidrug resistance pattern of extended spectrum β-lactamase (ESBL) producing <em>E. coli and Klebsiella </em>spp. isolates.</p> <p><strong>Methods</strong>: A descriptive analysis of 263 catheter tip specimens processed for culture and antimicrobial susceptibility testing was carried out in B&B Hospital, Lalitpur. Five different types of catheter tips were analyzed for microbiological growth and antimicrobial susceptibility testing.</p> <p><strong>Results</strong>: Among catheter tips, the highest percentage of microbial growth was observed in tracheostomy tip. Monomicrobial growth was recorded in 82.9% catheter tips and polymicrobial growth was observed in 17.1% tip samples. Of 180 isolates, gram negative rods (76.6%) followed by yeast (19.4%) and gram-positive cocci (3.9%) were isolated. Gram negative <em>Acinetobacter </em>spp. (25%) <em>and Pseudomonas </em>spp<em>. </em>(23.3%) and gram-positive <em>Enterococcus </em>spp. (2.2%) were the most frequently isolated bacteria. However, carbapenam was the most effective antibiotic for both groups.</p> <p><strong>Conclusion</strong>: Of the total isolates tested, 61.4% were found to be multidrug resistant (MDR). Among gram negative rods, 22.2% <em>E. coli </em>and 27.3% <em>Klebsiella </em>spp. were confirmed as ESBL producer. It is recommended to apply standard protocol during insertion and removal of catheter which may help in managing nosocomial infection associated with catheters.</p>}, journal={Tribhuvan University Journal of Microbiology}, author={Shrestha, Pushpa Man and Thapa, Nisha and Dahal, Navraj and Adhikari, Nabaraj and Shrestha, Upendra Thapa}, year={2018}, month={Sep.}, pages={32–38} }