@article{Neupane_K.C._Thakur_Panta_Joshi_Khanal_2019, title={Beta-Lactamases Production in Multi-drug Resistant Acinetobacter species Isolated from Different Clinical Specimens}, volume={6}, url={https://www.nepjol.info/index.php/tujm/article/view/26583}, DOI={10.3126/tujm.v6i0.26583}, abstractNote={<p><strong>Objectives:</strong> To determine the prevalence of <em>Acinetobacter</em> spp. from different clinical specimens and detect different types of β-lactamase enzymes.</p> <p><strong>Methods:</strong> Different clinical samples were collected and 125 <em>Acinetobacter</em> spp. were isolated.  Various biochemical tests were carried out to speciate the <em>Acinetobacter </em>spp. The antibiotic susceptibility pattern and β-lactamase enzymes like Extended spectrum β-lactamase (ESBL), Metallo β-lactamase (MBL) and AmpC β-lactamase were determined.</p> <p><strong>Results:</strong> Of the total 125 isolates, the most predominant species was <em>Acinetobacter calcoaceticus-A. baumannii</em> (Acb) complex (80%). Highest rate of isolation of <em>Acinetobacter</em> species were from in-patients (neonates’ blood sample).  Among all, 44.8% isolates were found to be MDR with the majority being resistant to aminoglycosides, carbapenems and fluoroquinolones but not to colistin. ESBL, MBL and AmpC beta-lactamase was detected in 43.2%, 15.2% and 1.6% of the isolates respectively.</p> <p><strong>Conclusion: </strong><em>Acinetobacter calcoaceticus-A. baumannii </em>complex should be considered for detection in hospitalized patients. The analysis of antibiotic susceptibility pattern and β-lactamases would be helpful to establish network surveillance in order to maintain and control the spread of these resistant strains.</p>}, journal={Tribhuvan University Journal of Microbiology}, author={Neupane, Mary and K.C., Sudeep and Thakur, Subash Kumar and Panta, Om Prakash and Joshi, Dev Raj and Khanal, Santosh}, year={2019}, month={Dec.}, pages={44–50} }