TY - JOUR AU - Tumbahangphe, Manita AU - Shrestha, Bijay Kumar AU - Shakya, Jenish AU - Khanal, Hemanta PY - 2019/12/07 Y2 - 2024/03/28 TI - Comparison of Biofilm Producing and Non-Producing Escherichia coli Isolated from Urine Samples of Patients Visiting a Tertiary Care Hospital of Morang, Nepal JF - Tribhuvan University Journal of Microbiology JA - TU J. Microbiol. VL - 6 IS - 0 SE - Articles DO - 10.3126/tujm.v6i0.26588 UR - https://www.nepjol.info/index.php/tujm/article/view/26588 SP - 76-81 AB - <p><strong>Objectives: </strong>The main objective of this study was to determine the prevalence of <em>Escherichia coli </em>among urinary tract infection (UTI) suspected patients visiting tertiary care hospital and to assess the biofilm producing ability of <em>E. coli</em> isolates.</p><p><strong>Methods: </strong>A prospective cross-sectional study was carried out in Biratnagar Metropolitan city, Eastern Nepal from December 2018 to May 2019. During the study 400 urine samples were collected from UTI suspected patients visiting a tertiary care hospital of Biratnagar. Urine samples were cultured by using semi-quantitative culture technique and identified. Antibiotic susceptibility testing was done by Kirby-Bauer Disk Diffusion method according to CLSI (2011) guidelines. Biofilm assays were performed by microtitre plate method.</p><p><strong>Results: </strong>This study reported 15% prevalence of <em>E. coli </em>out of 400 urine samples. 100% of <em>E. coli </em>isolates showed resistance to both Ampicillin and Amoxicillin while 100% were sensitive to Chloramphenicol. 70% (42/60) isolates were Multi Drug Resistance (MDR)<em>E. coli</em>. The maximum isolates (86.66%) were found to be biofilm producers by microtitre plate method. Resistance to other antibiotics such as Nalidixic acid (71.11% vs 46.66%), Norfloxacin (53.33% vs 46.66%), Cotrimoxazole (42.22% vs 26.66%) was comparatively higher among biofilm producers than non-biofilm producers. There was a significance of association between biofilm and MDR (p&lt;0.05).</p><p><strong>Conclusion: </strong>There is relation between the ability of biofilm formation and drug resistance in the bacterium resulting to the failure of antibacterial drugs.</p> ER -