Cytological and Biochemical Profile of Cerebrospinal Fluid from Meningitis Patients
Keywords:Bacterial meningitis, biochemical and cytological parameters, fungal meningitis
BACKGROUND: The term Meningitis is used to describe an inflammatory infection of the membranes surrounding the brain and spinal cord, which occurs as either a primary disease or secondarily to disease in some other part of the body. The diagnosis is primarily confirmed by analyzing cerebrospinal fluid (CSF). Early diagnosis of the cause may be based on the cytological and biochemical parameters. Our objective was to determine the cytological and biochemical profile of CSF from meningitis patients.
METHODS: In this cross sectional study, a total of 356 CSF specimens were collected from patients suspected of meningitis and processed microscopically and microbiologically by standard microbiological methods in Emergency Lab of Tribhuvan University Teaching Hospital (TUTH) Kathmandu, Nepal, over a period of six months, from March to August 2014 to determine cytological and biochemical parameters.
RESULTS: Out of the 13 confirmed bacterial meningitis cases from 356 processed CSF samples, the mean value of total leukocytes count (TLC) was found to be 337.3 cells/mm3 with predominant neutrophils (73.8%). The mean value of glucose and protein was 28.8 mg/dL and 89.4 mg/dL respectively in the cases of bacterial meningitis. Among the three confirmed cases of fungal meningitis, the mean value of TLC was 11.7 cells/mm3 with lymphocytic predominance. In fungal meningitis glucose level was found to be normal (45.0 mg/dL) with slight increase in protein (48.7 mg/dL).
CONCLUSIONS: Bacterial meningitis is generally characterized by increased TLC with predominance of neutrophils, decreased glucose and increased protein levels whereas fungal meningitis displays decreased TLC predominance of lymphocytes, normal or reduced glucose and slight increased protein level.
Ann. Clin. Chem. & Lab. Med. 1(1) 2015: 2-5
How to Cite
Authors who publish with this journal agree to the following terms:
- The author transfers copyright to the Nepalese Association for Clinical Chemistry.
- The journal publishes the work under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal and under the same share-alike license used here.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).