Expression of P53, PTEN and S100A4 in invasive ductal breast cancer and the clinical significance

Authors

  • Zhang Gang Hospital Of Hebei University,
  • Li Zhong Hospital Of Hebei University,
  • Lin Xiao-meng Hospital Of Hebei University, China.
  • Zhang Jun-hua Hospital Of Hebei University,
  • Cui Yong Hospital Of Hebei University,
  • Zhao Xing Hospital of Chengde Medical College,

DOI:

https://doi.org/10.3126/ajms.v6i6.12599

Keywords:

Invasive ductal breast cancer, P53, PTEN S100A4, Immunohistochemistry, Western blot

Abstract

Objective: To investigate the expression level of P53, PTEN and S100A4 in invasive ductal breast cancer and the clinical significance.

Methods: SP immunohistochemical and Western blot analysis were used to detect the expression of P53, PTEN and S100A4 protein in invasive ductal breast cancer, adjacent tissues and benign breast lesions. The results were aimed to explore the correlation of p53, PTEN, S100A4 and analyze the relationship between indicators and clinical pathology.

Results: Immunohistochemical results showed that the positive rates of p53 and S100A4 in invasive ductal breast cancer were 47.69% and 56.92%, which were significantly higher than that in benign breast lesions (16.00%, 22.00%) (p<0.05); The positive rates of PTEN in invasive ductal breast cancer was 24.62%, which were significantly lower than that in benign breast lesions (52.00%) (p<0.05); The expression of p53 was negatively correlated with PTEN in invasive ductal breast cancer (p<0.05). Western blot results revealed that the relative expression levels of p53, PTEN and S100A4 protein in invasive ductal breast cancer were 0.967±0.066, 0.785±0.044, 1.065±0.073, which were significant difference with that in benign breast lesions (0.549±0.032, 1.245±0.059, 0.370±0.065) (p<0.05); The expression of p53 has correlation with histological differentiating degree and lymph node metastasis (p<0.05), but it was irrelevant to the age, tumor size and clinical grade (p>0.05). The expression of S100A4 has correlation with clinical grade and lymph node metastasis (p<0.05), but no association with the age, tumor size and histological differentiating degree was observed in this study (p>0.05). The expressions of PTEN has correlation with lymph node metastasis p<0.05, but no association with the age, tumor size, clinical grade and histological differentiating degree was noted in our study (p>0.05).

Conclusion: Expression of P53, PTEN and S100A4 can be expected to be taken as to the prognosis biomarkers of breast cancer and potential targets for new and effective treatment strategies for breast cancer.

DOI: http://dx.doi.org/10.3126/ajms.v6i6.12599

Asian Journal of Medical Sciences Vol.6(6) 2015 43-47

Downloads

Download data is not yet available.
Abstract
715
PDF
654

Downloads

Published

2015-06-10

How to Cite

Gang, Z., Zhong, L., Xiao-meng, L., Jun-hua, Z., Yong, C., & Xing, Z. (2015). Expression of P53, PTEN and S100A4 in invasive ductal breast cancer and the clinical significance. Asian Journal of Medical Sciences, 6(6), 43–47. https://doi.org/10.3126/ajms.v6i6.12599

Issue

Section

Original Articles