Interleukin-1 involved in Apoptosis of Beta-Thalassemia/Hemoglobin E Erythroid Progenitor Cells

Authors

  • Umesh Prasad Gupta Department of Medical Laboratory Science, Faculty of Science and Technology, Pokhara University
  • Pranee Fucharoen Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakhon, Pathom
  • Dalina I Tanyong Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Bangkok

DOI:

https://doi.org/10.3126/ajms.v3i4.6805

Keywords:

Ineffective erythropoiesis, apoptosis, IL-1, beta-thalassemia, HbE

Abstract

Objective: The major pathophysiological features of β-thalassemia are anemia and ineffective erythropoiesis. Ineffective erythropoiesis has been shown by an intense marrow erythroid hyperplasia and increased apoptosis during basophilic to orthochromatic normoblast stages. Some cytokines like interferon-γ (IFN-γ), tumor necrosis factor α (TNF-α) and interleukin-1 (IL-1) have been found to involved in apoptosis. Although the pro-apoptotic activity of IFN-γ and TNF-α is well documented, there are only few studies on IL-1, especially on erythroid lineage. In this in vitro study, the role of cytokine IL-1α and IL-1β in apoptosis of erythroid progenitor cells from β- thalassemia/HbE patients was assessed.

Methods: Erythroid progenitor cells were isolated from peripheral blood of healthy subjects and β-thalassemia/HbE patients. Cells were then cultured, with and without 20 ng/ml IL-1α and IL-1β. Total cells and percent cell viability were performed by using trypan blue staining. Percent cell apoptosis was analyzed by using flow cytometer.

Results: Both IL-1α and IL-1β were significantly decreased erythroid progenitor cells. IL-1 at 20 ng/ml reduced the glycophorin A positive cells and percent cell viability of erythroid progenitor cells from β-thalassemia/HbE patients, while there was increased apoptosis in this group. The highest percent apoptosis was observed in 20 ng/ml IL-1β treated β-thalassemia/HbE erythroid progenitor cells.

Conclusion: IL-1β could be involved in apoptosis of erythroid progenitor cells from β-thalassemia/HbE patients which might be related with ineffective erythropoiesis of the disease.

DOI: http://dx.doi.org/10.3126/ajms.v3i4.6805

Asian Journal of Medical Science Vol.3(4) 2012 pp.8-14

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Published

2013-04-08

How to Cite

Gupta, U. P., Fucharoen, P., & Tanyong, D. I. (2013). Interleukin-1 involved in Apoptosis of Beta-Thalassemia/Hemoglobin E Erythroid Progenitor Cells. Asian Journal of Medical Sciences, 3(4), 8–14. https://doi.org/10.3126/ajms.v3i4.6805

Issue

Vol. 3 No. 4 (2012)

Section

Original Articles

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