Antibiotic susceptibility pattern of biofilm forming uropathogenic Escherichia coli isolated from UTI infected patients of Koshi Zonal Hospital in Biratnagar, Nepal

  • S Chaudhary Department of Microbiology, Mahendra Morang Adarsh Multiple Campus, Biratnagar
  • B Khatiwada Department of Microbiology, Mahendra Morang Adarsh Multiple Campus, Biratnagar
  • N K Chaudhary Department of Chemistry, Mahendra Morang Adarsh Multiple Campus, Biratnagar
Keywords: Biofilm, urinary tract infection, antibiotic susceptibility, E. coli, multidrug resistance.

Abstract

Objectives: To investigate the prevalence and antibiotic resistance pattern of biofilm-forming Uropathogenic Escherichia coli (UPEC) from urine samples isolated from UTI infected patients of Koshi zonal hospital, Biratnagar.

Methods: A total of 51 urine samples from urinary tract infected patients were collected from Koshi zonal hospital, Biratnagar in the period of July to August 2017. Following the isolation and identification of biofilm-forming uropathogenic Escherichia coli, antibiotic susceptibility test was performed by a modified Kirby-Bauer disc diffusion technique. The biofilm detection was done by Congo red agar method.

Results: In the present study, 45% of the urine samples showed a predominant growth of E. coli, among which 70% of isolates exhibited positive biofilm formation. Biofilm forming isolates revealed 100%, 87.5%, 75%, 63% and 12.5% resistant to erythromycin, amoxicillin, cefotaxime, levofloxacin, and nitrofurantoin respectively. Approximately 87.5% of biofilm-forming isolates were found multi-drug resistant.

Conclusion: The study revealed the major issue of UTI by E. coli which may be due to poor sanitation, not the proper cleanliness of genitals and unsafe sexual intercourse. Nitrofurantoin and levofloxacin were examined the most effective antibiotics for UPEC. 

BIBECHANA 16 (2019) 47-54 

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Abstract
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Published
2018-11-22
How to Cite
Chaudhary, S., Khatiwada, B., & Chaudhary, N. (2018). Antibiotic susceptibility pattern of biofilm forming uropathogenic Escherichia coli isolated from UTI infected patients of Koshi Zonal Hospital in Biratnagar, Nepal. BIBECHANA, 16, 47-54. https://doi.org/10.3126/bibechana.v16i0.19192
Section
Research Article