Partial Proteome Map of Campylobacter Jejuni Strain Nctc11168 by Gel-Free Proteomics Analysis

  • Zilun Shi The Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln 7647
  • Chris Dawson The Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln 7647
  • Stephen L.W. On The Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln 7647
  • Malik Altaf Hussain The Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln 7647
Keywords: foodborne pathogen, Campylobacter jejuni, iTRAQ analysis, protein functions, proteomics, proteome

Abstract

A proteome map of the foodborne pathogen Campylobacter jejuni NCTC11168 was analyzed using a state-of-the-art gel-free proteomic approach for the first time. A whole cell protein extract was prepared from the C. jejuni strain NCTC11168 grown in brain heart infusion (BHI) broth at 42°C under microaerobic conditions. A gel-free technique using isobaric tags for relative and absolute quantitation (iTRAQ) was employed to create a protein expression profile of the strain. Liquid chromatography-mass spectrometry (LC-MS/MS) was used to identify the proteins. Protein functionalities were searched to classify them. A total of 235 proteins were identified in the whole cell protein fraction of C. jejuni NCTC11168 cells using iTRAQ analysis. Functional grouping of the identified proteins showed that forty percent of these proteins were associated with energy metabolism, protein synthesis and genetic information processing. iTRAQ was faster, easier and proved more sensitive than two-dimensional gel-based proteomics approaches previously applied to C. jejuni, making it an attractive tool for further studies of cellular physiological response.

DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11253  

Int J Appl Sci Biotechnol, Vol. 2(4): 464-477

 

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Abstract
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Published
2014-12-25
How to Cite
Shi, Z., Dawson, C., On, S., & Hussain, M. (2014). Partial Proteome Map of Campylobacter Jejuni Strain Nctc11168 by Gel-Free Proteomics Analysis. International Journal of Applied Sciences and Biotechnology, 2(4), 464-477. https://doi.org/10.3126/ijasbt.v2i4.11253
Section
Research Articles