Molecular Cloning, Expression and FPLC Purification of Lectin A from Pseudomonas aeruginosa

  • Peyman Ghoraishizadeh Faculty of Medicine, Laboratory of Stem Cells and nano-regenerative medicine, Universidad de los Andes, Santiago
  • Shraddha Raikar Manipal School of Regenerative Medicine, Manipal University, Bangalore
  • Mahsa Takhtechian Indian Academy College, Bangalore University, Bangalore
Keywords: Molecular Cloning, Lectin A, Pseudomonas aeruginosa

Abstract

Pseudomonas aeruginosa (PA) as an opportunistic pathogen infects the pulmonary tract, bladder, cystic fibrosis patients and burn victims. PA infections treatment is challenging because of its ability to rapidly develop resistance to multiple classes of antibiotics. Lectin is protein that isexpressed in cell of PA and cause of infection by attaching to the host cells. Lectin A gene coding lectin protein so we cloned and expressedthis gene then purified of related protein, that can be used in preparation of vaccine to treat PA infections.

DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11559

Int J Appl Sci Biotechnol, Vol. 2(4): 529-536

 

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Abstract
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Published
2014-12-25
How to Cite
Ghoraishizadeh, P., Raikar, S., & Takhtechian, M. (2014). Molecular Cloning, Expression and FPLC Purification of Lectin A from Pseudomonas aeruginosa. International Journal of Applied Sciences and Biotechnology, 2(4), 529-536. https://doi.org/10.3126/ijasbt.v2i4.11559
Section
Research Articles