Kinetics of acetylcholinesterase inhibition by an aqueous extract of Cuminum cyminum seeds.

  • Suresh Kumar GGS Indraprastha University, New Delhi
  • Suman Chowdhury GGS Indraprastha University, New Delhi
Keywords: Acetylcholinestrase, Cumin cyminum, kinetics, Alzheimer’s diseases

Abstract

The cholinergic hypothesis of Alzheimer’s disease (AD) has provided the rationale for the current pharmacotherapy of this disease. Acetylcholinesterase (AChE) inhibitors are currently the only approved therapy for the symptomatic treatment of AD. The current drugs available in the market has shown various side effect which prompted scientist to search for new and potent AChE inhibitors which exerts minimal side effect in AD patient. In present study, an aqueous extract of Cumin cyminum was tested for in vitro acetylcholinesterase inhibitory activity based on Ellman’s method. C. cyminum showed maximum inhibition of 76.90±0.003% in an aqueous extract at 50μg/ml final concentration. Further studies were conducted to elucidate the mode of AChE inhibition by kinetic studies. Competitive inhibition was observed at lower concentrations (12.5μg/ml & 25μg/ml) and mixed inhibition was observed at higher concentrations (50μg/ml & 100μg/ml).

DOI: http://dx.doi.org/10.3126/ijasbt.v2i1.9348  

Int J Appl Sci Biotechnol, Vol 2(1): 64-68

Downloads

Download data is not yet available.
Abstract
542
PDF
211

Author Biographies

Suresh Kumar, GGS Indraprastha University, New Delhi
Associate Professor, University School of Biotechnology.
Suman Chowdhury, GGS Indraprastha University, New Delhi
Junior Reasearch Fellow, University School of Biotechnology.
Published
2014-03-25
How to Cite
Kumar, S., & Chowdhury, S. (2014). Kinetics of acetylcholinesterase inhibition by an aqueous extract of Cuminum cyminum seeds. International Journal of Applied Sciences and Biotechnology, 2(1), 64-68. https://doi.org/10.3126/ijasbt.v2i1.9348
Section
Research Articles